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pST_PvLEA4_repeats_k5_1 (pBS0766)

Plasmid

#185247

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k5_1

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k4_14 (pBS0764)

Plasmid

#185246

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k4_14. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k4_14

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k4_1 (pBS0763)

Plasmid

#185245

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k4_1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k4_1

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k3_11 (pBS0761)

Plasmid

#185243

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_11. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_11

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DrHD_dom3_k5_0 (pBS0758)

Plasmid

#185242

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k5_0. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DrHD_dom3_k5_0

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DrHD_dom3_k3_7 (pBS0755)

Plasmid

#185241

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_7. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DrHD_dom3_k3_7

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DrHD_dom3_k3_1 (pBS0754)

Plasmid

#185240

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DrHD_dom3_k3_1

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_CI016_HUMAN (pBS0747)

Plasmid

#185238

Purpose

For the mammalian expression of the human protein CI016_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CI016_HUMAN

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_AdHsAPO-44 (pBS0746)

Plasmid

#185237

Purpose

For the mammalian expression of the Chinese giant salamander and human fusion protein AdHsAPO-44. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AdHsAPO-44

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_AdAPOA4-44 (pBS0745)

Plasmid

#185236

Purpose

For the mammalian expression of the Chinese giant salamander protein AdAPOA4-44. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AdAPOA4-44

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOA4_ANDDA_52-358 (pBS0744)

Plasmid

#185235

Purpose

For the mammalian expression of the Chinese giant salamander protein APOA4_ANDDA_52-358. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOA4_ANDDA_52-358

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pS97splitRBP_S1-Enz

Plasmid

#183767

Purpose

Expresses SHIP1-Enzyme domain flanked by RBP

Depositor

William Kerr

Article

Sudan et al Front Immunol. 2022 May 4;13:830961. doi

Insert

SHIP1 (INPP5D Human)

Tags

6HIS, RBP (C-terminal) w/HRV3C cleavage site, and…

Expression

Bacterial

Mutation

includes aa N393 to aa L864 only

Promoter

T7lac

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pS97splitRBP_S2-Enz

Plasmid

#183768

Purpose

Expresses SHIP2-Enzyme domain flanked by RBP

Depositor

William Kerr

Article

Sudan et al Front Immunol. 2022 May 4;13:830961. doi

Insert

SHIP2 (INPPL1 Human)

Tags

6HIS, RBP (C-terminal) HRV3C cleavage site, and R…

Expression

Bacterial

Mutation

includes aa K414 to aa V872 only

Promoter

T7lac

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET24TEV-tSHIP2

Plasmid

#183771

Purpose

Expresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backbone

Depositor

William Kerr

Article

Sudan et al Front Immunol. 2022 May 4;13:830961. doi

Insert

SHIP2 (INPPL1 Human)

Tags

6HIS and Tobacco Etch Virus (TEV) Cleavage site

Expression

Bacterial

Mutation

includes aa18 to aa985 only

Promoter

T7 lac

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)-ABL1-gRNA1-exon4

Plasmid

#163322

Purpose

eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 1_Exon4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)-ABL1-gRNA2-exon4

Plasmid

#163323

Purpose

eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 2_Exon4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
pLKO.1P RTEL1_1

Plasmid

#160799

Purpose

Suppress RTEL1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shRTEL1_1

Use

Lentiviral

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1B CDC25A_2

Plasmid

#160768

Purpose

Suppress CDC25A

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25A_2

Use

Lentiviral

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1P RTEL1_2

Plasmid

#160800

Purpose

Suppress RTEL1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shRTEL1_2

Use

Lentiviral

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P PRIM2_2

Plasmid

#160796

Purpose

Suppress PRIM2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPRIM2_2

Use

Lentiviral

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge