We narrowed to 5,332 results for: cycs
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pGC480
Plasmid#42600DepositorInsertlet-363 cDNA
UseRNAiTagsExpressionMutationPromoterT7Available sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUCdeltaSS-ERE
Plasmid#108217PurposeYeast reporter gene with estrogen response element (ERE) upstream of CYC1 promoter driving lacZ expressionDepositorInsertERE-CYC1-lacZ
UseTagsExpressionYeastMutationPromoterCYC1Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBB328
Plasmid#221533PurposePlasmid for the introduction of Mariner transposon that inserts deoxyviolacein cassette behind indigenous promoters in target bacterial strain. Plasmid has tetracycline marker for selection.DepositorInsertsvioABCE
Mariner transposase and transposon backbone from pBB298 (originally from pEB001)
Tetracycline antibiotic cassette from pRK415
UseTagsExpressionBacterialMutationPromoterNone, contains RBS siteAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDT4
Plasmid#199120Purposescrambled promoter circular DNA templateDepositorInsertsynthetic transcription template
UseTagsExpressionBacterialMutationmutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …PromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE30-butelase1
Plasmid#115430PurposeExpresses the macrocyclizing asparaginyl endopeptidase butelase 1 in E. coliDepositorInsertbutelase 1
UseTags6-HisExpressionMutationPromoterT5Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCVD438
Plasmid#63759Purposeeae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.DepositorInserteae
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT2iU6m5Hoxa13bE4PCh(#765)
Plasmid#184102Purposecyclofen-inducible fish Hoxa13b activation via zebrafish transient transgenesis or mRNA synthesisDepositorInsert5myc-ZfHoxa13b-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes) + in vitro tra…Tags5xMycExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-Ubi+SP6Available sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDT2
Plasmid#199119Purposecircular transcription templateDepositorInsertlambda PR' - repeat cassette - E. coli rpoB - lambda TR'
UseTagsExpressionBacterialMutationmutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …PromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT22U6CatalE4PCh(#767)
Plasmid#184078Purposecyclofen-inducible Catalase activation in zebrafish permanent transgenicDepositorInsertCatalDeltaC-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes) + in vitro tra…TagsExpressionMutationCatalDeltaC: deleted 516-527; ERT2: G400V, M543A,…PromoterZf-Ubi+SP6Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasH3En2SE4P3Che(#1212)
Plasmid#184098Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert3HA-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags3xHAExpressionMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…Promoter5xUASAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasm5En25EE4P3Che(#1213)
Plasmid#184099Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(5E)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationchkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …Promoter5xUASAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasm5En2SE4P3Che(#1209)
Plasmid#184097Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…Promoter5xUASAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22cUm5En2SE4P3Che(#1189)
Plasmid#184095Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22cUm5En2E4P3Che(#1188)
Plasmid#184094Purposecyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutation5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasm5En2E4P3Che(#1208)
Plasmid#184096Purposecyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutation5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …Promoter5xUASAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
tFucci(CA)5
Plasmid#153521PurposeNew color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.DepositorInserttFucci(CA)5.
UseTagsAzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)ExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
tFucci(SA)5
Plasmid#153520PurposeNew color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.DepositorInserttFucci(SA)5.
UseTagsAzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)ExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfpC
Plasmid#111614PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only
