We narrowed to 144,455 results for: ins

1. pCRISPaint-3xP3-RFP

   Plasmid

   #130276

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   3xP3-RFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPaint-sfGFP-3xP3-RFP

   Plasmid

   #127566

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sfGFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFD3-frame_selector_2

   Plasmid

   #127555

   Purpose

   Drosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-in

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sgRNA frame 2
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6:3

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIK127

   Plasmid

   #65631

   Purpose

   Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.

   Depositor

   Iskra Katic

   Article

   Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

   Insert

   Peft-3::gfp::h2b::tbb-2 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. FR_GFP

   Plasmid

   #31302

   Depositor

   Gerhart Ryffel

   Article

   Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

   Insert

   green fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-eGFP-P2A-dTAG-Ing3

   Plasmid

   #170754

   Purpose

   Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

   Depositor

   Kristian Helin

   Article

   Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

   Insert

   eGFP-P2A-2xHA-dTAG
   

   Use

   Mouse Targeting

   Tags

   eGFP-P2A-2xHA-dTAG

   Expression

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   none

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPaint-T2A-Gal4-3xP3-RFP

   Plasmid

   #127556

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Gal4
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPaint-T2A-FLPo-3xP3-RFP

   Plasmid

   #127561

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   FLPo
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19_dTAG-KANSL3-targeting

   Plasmid

   #170755

   Purpose

   Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

   Depositor

   Kristian Helin

   Article

   Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

   Insert

   BSD-P2A-2xHA-dTAG
   

   Use

   Mouse Targeting

   Tags

   BSD-P2A-2xHA-dTAG

   Expression

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPaint-T2A-sfGFP-3xP3-RFP

    Plasmid

    #127557

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sfGFP
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPaint-T2A-Gal80-3xP3-RFP

    Plasmid

    #127562

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Gal80
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRISPaint-T2A-Gal4DBD-3xP3-RFP

    Plasmid

    #127564

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Gal4DBD
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPaint-T2A-p65-3xP3-RFP

    Plasmid

    #127565

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    p65
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPaint-T2A-QF2-3xP3-RFP

    Plasmid

    #127559

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    QF2
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPaint-T2A-Nluc-3xP3-RFP

    Plasmid

    #127563

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Nluc
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIK198

    Plasmid

    #65629

    Purpose

    "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPaint-T2A-LexGAD-3xP3-RFP

    Plasmid

    #127558

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    LexGAD
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmrROSA-DEST

    Plasmid

    #86953

    Purpose

    Gateway destination vector for targeting the ROSA locus for conditional mouse knock-ins.

    Depositor

    Joseph Dougherty

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSPObooster

    Plasmid

    #216160

    Purpose

    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

    Depositor

    Grant Brown

    Article

    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

    Inserts

    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    MKT1 gene containing an aspartic acid to glycine …

    Promoter

    endogenous promoter

    Available since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAGT9050

    Plasmid

    #219429

    Purpose

    PapE Exonuclease fused to linker 4LF2 as N-terminal tag module (AATG_PapE-4*LF2_AGGT)

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    AATG_PapE-4xLF2_AGGT
    

    Use

    Moclo compatible level 0 module

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge