We narrowed to 33,308 results for: grn

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pT7-SpCas9_sgRNA-site2 (RTW448)

Plasmid

#160137

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-SpCas9_sgRNA-site2 (RTW448)

Plasmid

#160137

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-scramble

Plasmid

#62285

Purpose

expression of scramble sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

scramble sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-scramble

Plasmid

#62285

Purpose

expression of scramble sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

scramble sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [n] (GB1207)

Plasmid

#75408

Purpose

tRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [n]

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [n] (GB1207)

Plasmid

#75408

Purpose

tRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [n]

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9-Puro HLAG-2B-sgRNA

Plasmid

#182552

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Article

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2B-sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9-Puro HLAG-2B-sgRNA

Plasmid

#182552

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Article

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2B-sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA SOX17 -91

Plasmid

#50923

Purpose

U6 driven sgRNA targeting Sox17 -91 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Sox17 -91 sgRNA (SOX17 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA SOX17 -91

Plasmid

#50923

Purpose

U6 driven sgRNA targeting Sox17 -91 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Sox17 -91 sgRNA (SOX17 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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gRNAs[bTub+Sxl].1046B

Plasmid

#112692

Purpose

express two gRNA targeting bTub & Sxl under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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gRNAs[bTub+Sxl].1046B

Plasmid

#112692

Purpose

express two gRNA targeting bTub & Sxl under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP

Plasmid

#89061

Purpose

AAV-gRNA targeting the murine Ldlr gene

Depositor

William Lagor

Article

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

Chicken beta actin with partial CMV enhancer

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP

Plasmid

#89061

Purpose

AAV-gRNA targeting the murine Ldlr gene

Depositor

William Lagor

Article

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

Chicken beta actin with partial CMV enhancer

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9wt)-Rif1

Plasmid

#122306

Purpose

Expresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Rif1 (Rif1 Synthetic)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9wt)-Rif1

Plasmid

#122306

Purpose

Expresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Rif1 (Rif1 Synthetic)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNAmreB

Plasmid

#149594

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNAmreB

Plasmid

#149594

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Eif4a1-R

Plasmid

#122345

Purpose

Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Eif4a1

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Eif4a1-R

Plasmid

#122345

Purpose

Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Eif4a1

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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