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Plasmid#159107PurposeThe SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).DepositorInsertNSP12
TagsHis-SUMO tagExpressionBacterialMutationNonePromoterT7Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST p38KTRClover
Plasmid#59152PurposeLentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertp38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
UseLentiviralExpressionMammalianPromoterPGKAvailable SinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-NbVHH05-ALFA-His
Plasmid#171569PurposeBacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)DepositorInsertNbVHH05-ALFA-His (UBE2J1 Human)
TagsALFA-tag/His-tag and PelB signal peptideExpressionBacterialAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
WRN scFv [N428/12]
Plasmid#206784PurposeMammalian Expression of WRN scFV. Derived from hybridoma N428/12 scFv.DepositorAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lrrc4-Fc-His
Plasmid#72084PurposeExpresses the extracellular region of the LRRC4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-his-3C_Tau352
Plasmid#100092PurposeExpresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tagDepositorInsertFetal microtubule-associated protein tau (MAPT Human)
TagsHis6ExpressionBacterialMutationwild type sequencePromoterT7Available SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)
Plasmid#229817PurposeA gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.DepositorTypeEmpty backboneUseTol2 destination vectorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTRClover
Plasmid#59151PurposeLentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertJNK Kinase Translocation Reporter (MAPK8 Mouse, Human)
UseLentiviralExpressionMammalianPromoterPGKAvailable SinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-EphB3-SV40-GFP (B3G)
Plasmid#65443PurposeExpression of EPHB3 and GFP in mammalian cellsDepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-eGFP-hcGAS
Plasmid#108674PurposeFor production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged human cGASDepositorAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Flrt2-myc
Plasmid#72192PurposeExpress full-length Flrt2with a C-terminal myc tagDepositorAvailable SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DYRK1A(K188R)-2A-mCherry
Plasmid#177166PurposeExpresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Lysine at position 188 substituted with Arginine.DepositorInsertdual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
UseAAVTags2A peptide and mCherryExpressionMammalianMutationChanged Lysine 188 to ArgininePromoterCMV with beta globin intronAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMG024 MBP-GSK3β-HA-His, GST_λPPase
Plasmid#196182PurposeCo-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-HisDepositorAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcsII-pb5-flag
Plasmid#86769PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertproteasome beta subunit 5 (PSMB5 Human)
UseLentiviralTagsflagExpressionMammalianPromoterEF-1aAvailable SinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Mm2
Plasmid#197100PurposeTo express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivatiDepositorInsertPylRS variant, Pyl tRNA, kanamycin resistance gene
ExpressionBacterialMutationLys61, Glu131, Ala306, Phe384, Glu444 (PylRS)Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVC-FUS-WT
Plasmid#175066Purposeexpresses mVenus and mCherry tagged to FUS wildtype in mammalian cellsDepositorInsertFUS glycine rich protein (FUS Human)
TagsmVenus + mCherryExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala
Plasmid#208172PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
TagsStrep-tagExpressionMammalianMutationArg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…Available SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala
Plasmid#208173PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
TagsStrep-tagExpressionMammalianMutationLeu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…Available SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala
Plasmid#208174PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
TagsStrep-tagExpressionMammalianMutationLys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…Available SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MARK2-K82H-CR
Plasmid#229425PurposeLentiviral or overpexression of cDNADepositorInsertMARK2-K82H (MARK2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationKinase-dead mutant K82H; silent mutation CRISPR r…PromoterEFSAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only