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pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS

Plasmid

#128833

Purpose

Knock-in of nuclear EYFP into mouse Elf5 locus

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Nuclear EYFP into Elf5 locus (Elf5 Mouse)

Use

Mouse Targeting

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

Plasmid

#163572

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-NG-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PPO-Venus

Plasmid

#139502

Purpose

Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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LMPd Ametrine Got2 shRNA#3

Plasmid

#220593

Purpose

Retroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Ma et al bioRxiv [Preprint]. 2023 Jun 11:2023.06.

Insert

Got2 shRNA (Got2 Mouse)

Use

Retroviral

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CoV2-Omicron BA.1 spike

Plasmid

#194602

Purpose

Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

Depositor

Ben Murrell

Article

Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

Insert

CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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VARP GFP-pLXIN

Plasmid

#62950

Purpose

full length human VARP with C-terminal EGFP tag cloned into pLXIN

Depositor

Paul Luzio

Article

Hesketh et al Dev Cell. 2014 Jun 9;29(5):591-606. doi:

Insert

VARP (ANKRD27 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

silent mutations of amino acids 422-428 to produc…

Promoter

LTR

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pGEX SHP-2(N)-SH2

Plasmid

#46498

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(N) (PTPN11 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-104

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a1MKK7B2

Plasmid

#19731

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

Jnk1a1MKK7b2 (Map2k7, MAPK8 Mouse, Human)

Tags

Flag

Expression

Mammalian

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
pGEX Abl-SH2

Plasmid

#46402

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Abl (ABL1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 120-225

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mScarlet-gamma9

Plasmid

#140097

Purpose

Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

GNGT2

Tags

N-terminal mScarlet tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP C1-Eps8 ∆cap

Plasmid

#74786

Purpose

mammalian expression of the capping activity mutant Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

Insert

Eps8 (Eps8 Mouse)

Expression

Mammalian

Mutation

V689D, L693D

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pCSCRET2(#323)

Plasmid

#184058

Purpose

cyclofen-inducible CRE activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

Insert

6myc-CRE-ERT2

Use

Synthetic Biology

Tags

6xMyc

Expression

Mammalian

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

sCMV+SP6

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CBA-hGBA1-cm-hGBA1-hGHpA

Plasmid

#218795

Purpose

AAV reporter genome plasmid

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

CMV-CBA-hGBA1-cm-hGBA1-hGHpA

Use

AAV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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R620-M15-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 D614G

Plasmid

#170201

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) D614G (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

D614G

Promoter

CMV51

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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3322_pETcon_SARS2_Omicron-BA2

Plasmid

#184409

Purpose

yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B

Plasmid

#203695

Purpose

expresses ATP10B

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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TRCN0000072225

Plasmid

#78160

Purpose

shLacZ control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

LacZ

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP mScarlet-MOSPD2 W201E

Plasmid

#186477

Purpose

Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX RASGAP(N)-SH2

Plasmid

#46432

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Gap/RASGAP(N) (RASA1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 168-283

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cas9-T2A-STOP-TdT

Plasmid

#126425

Purpose

Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-T2A-STOP-TdT (DNTT Streptococcus pyogenes, Human)

Expression

Mammalian

Mutation

alanine-6 of TdT has been changed to a STOP codon

Promoter

pCMV

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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