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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,501 results for: LEA

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Showing: 10241 - 10260 of 18501 results

  8. pCLASP_Crz1(19A)_CLASP_RGS2membrane

    Plasmid
    #133085
    Purpose
    Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.
    Depositor
    Hana El-Samad
    Publication
    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2
    Insert
    Crz1*-CLASP
    Expression
    Bacterial and Yeast
    Promoter
    pADH1
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    133085-plasmid-map-sequence-id-268911

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  11. cycLuc_SuMMVs

    Plasmid
    #119210
    Purpose
    Circularly permutated firefly luciferase with SuMMVs cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Publication
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cyclic luciferase with SuMMVp cleavage site
    Use
    Luciferase
    Tags
    AU1 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119210-plasmid-map-sequence-id-232040

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  12. pCMV-NFIB2ERt2

    Plasmid
    #112703
    Purpose
    Expression of NFIB2-ERT2 fusion protein
    Depositor
    Richard Gronostajski
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclear Factor I B2 (Nfib Mouse)
    Tags
    ERT2 and HA
    Expression
    Mammalian
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112703-plasmid-map-sequence-id-214845

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  13. pSV40_HA-mPACT

    Plasmid
    #120537
    Purpose
    protein expression
    Depositor
    Petr Svoboda
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PACT (Prkra Mouse)
    Expression
    Mammalian
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120537-plasmid-map-sequence-id-233981

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  14. pOCC110

    Plasmid
    #118905
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and LPETGg
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118905-plasmid-map-sequence-id-232327

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  15. pOCC215

    Plasmid
    #118879
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
    Tags
    Dendra2, HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118879-plasmid-map-sequence-id-232264

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  19. tdEos-CENPA-C-22

    Plasmid
    #57597
    Purpose
    Localization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    CENPA (CENPA Human)
    Tags
    tdEos
    Expression
    Mammalian
    Mutation
    Please see Depositor Comments
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57597-plasmid-map-sequence-id-228931

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  20. Cwc25-pRSETA

    Plasmid
    #61043
    Purpose
    Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.
    Depositor
    Nils Walter
    Publication
    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145
    Insert
    Cwc25
    Tags
    TEV site, Histidine Tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 10241 - 10260 of 18501 results