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pCDNA3.1-Tmem98deltaN-FLAG

Plasmid

#124502

Purpose

Expresses an N-terminally truncated version of FLAG-tagged mouse TMEM98 in mammalian cells (C-terminal tag)

Depositor

Renee van Amerongen

Publication

van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

Insert

Tmem98 (Tmem98 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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SNX14-PX (561-686)

Plasmid

#119095

Purpose

Bacterial expression of human phox homology (PX) domain, SNX14-PX (561-686)

Depositor

Brett Collins

Publication

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX14-PX (561-686) (SNX14 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAEF6

Plasmid

#136303

Purpose

pAEF5 plasmid with two gRNA inserted

Depositor

Gilles Fischer

Publication

Agier et al Methods Mol Biol. 2021;2196:181-198. doi

Insert

double cassette coding for gRNA

Use

CRISPR; Template for pcr to insert double grna co…

Available since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRS313-PRP8

Plasmid

#90964

Purpose

yeast vector

Depositor

Christine Guthrie

Publication

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Promoter

native

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAc-Cdc3-Cdc10

Plasmid

#131160

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Publication

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

Cdc3 + Cdc10

Use

Bicistronic

Tags

Cdc10: S Tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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FB012

Plasmid

#119709

Purpose

Complete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Publication

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

PtrpC:hph:Ttub

Use

Synthetic Biology; Domestication of dna parts for…

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pFOXO4.1.0-gDNA

Plasmid

#112408

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXO4

Depositor

Kevin White

Insert

FOXO4 (FOXO4 Human)

Use

CRISPR

Expression

Mammalian

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGex4t2 with full-length SUUR

Plasmid

#112990

Purpose

Expresses fusion of GST and full-length Drosophila SUUR

Depositor

Terry Orr-Weaver

Publication

Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

Insert

SuUR (SuUR Fly)

Expression

Bacterial

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-Y100F

Plasmid

#110826

Purpose

Bacterial expression of E.coli DHFR Y100F mutant

Depositor

Stephen Benkovic

Publication

Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

Insert

ecDHFR

Expression

Bacterial

Mutation

Y100 mutated to F100

Promoter

T7

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 1-225 (#431)

Plasmid

#99927

Purpose

mammalian expression

Depositor

James Brugarolas

Publication

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

C-terminal deletion; encodes AA 1-225

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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YIp204-P30-His-POL30

Plasmid

#99550

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA); TRP1 marker

Depositor

Helle Ulrich

Publication

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

POL30

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-M2/X184W-IRES(isoform 1)

Plasmid

#99560

Purpose

mammalian expression of mutant TMEM230 (isoform 1) and ZsGreen

Depositor

Han-Xiang Deng

Publication

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Expression

Mammalian

Mutation

M2/X184W

Promoter

CMV

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-M1/R141L-IRES(isoform 1)

Plasmid

#99559

Purpose

mammalian expression of mutant TMEM230 (isoform 1) and ZsGreen

Depositor

Han-Xiang Deng

Publication

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Expression

Mammalian

Mutation

M1/R141L

Promoter

CMV

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Munc18cF119E-pDEST27

Plasmid

#85625

Purpose

GST tagged Munc18c with a F119E mutation

Depositor

David James

Publication

Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

Insert

Munc18c (Stxbp3 Mouse)

Tags

GST

Expression

Mammalian

Mutation

F119E

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;cldn5bCE1-P1Egfp

Plasmid

#90154

Purpose

contains cldn5b enhancer sequence, and cldn5b specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Publication

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

cldn5bE1P1Egfp (cldn5b Zebrafish)

Tags

EGFP

Expression

Bacterial

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NR2F6_2

Plasmid

#86343

Purpose

Encodes gRNA for 3' target of human NR2F6

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against NR2F6 (NR2F6 Human)

Use

CRISPR

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NR2F6_1

Plasmid

#86342

Purpose

Encodes gRNA for 3' target of human NR2F6

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against NR2F6 (NR2F6 Human)

Use

CRISPR

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSico shMAPKAPK3 human

Plasmid

#85661

Purpose

expression of shRNA targeting human MAPKAPK3

Depositor

Julien Sage

Publication

Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

Insert

MAPKAPK3 (MAPKAPK3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_huMcl-1.2

Plasmid

#85531

Purpose

Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporter

Depositor

Marco Herold

Publication

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

hu Mcl-1.2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1t

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCMB-PERr

Plasmid

#61203

Purpose

Fluorescent reporter for peroxisomes mCherry-SKL expressed under MtBCP1 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

mCherry-SKL

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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