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pENTR-D-kcnj10b

Plasmid

#173138

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Publication

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj10b (LOC100329607 Zebrafish)

Use

Pcr cloning vector

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E18: attB4-polyhedrinp>-attB5

Plasmid

#162977

Purpose

Gateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r

Plasmid

#162937

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6 fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E01: attB4-polyhedrinp>His6-nostop-attB1r

Plasmid

#162932

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6 fusion with AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 LbCas12a (GB1439)

Plasmid

#160573

Purpose

Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

Depositor

Diego Orzaez

Publication

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

LbCas12a

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 AsCas12a (GB1438)

Plasmid

#160572

Purpose

Human codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).

Depositor

Diego Orzaez

Publication

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AsCas12a

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnt2a

Plasmid

#164963

Purpose

Zebrafish kcnt2a gene CDS

Depositor

GuangJun Zhang

Publication

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnt2a (kcnt2 Zebrafish)

Use

Pcr cloning vector

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnma1b

Plasmid

#164952

Purpose

Zebrafish kcnma1b gene CDS

Depositor

GuangJun Zhang

Publication

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnma1b (kcnma1b Zebrafish)

Use

Pcr cloning vector

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-N342W

Plasmid

#160536

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-N342W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, N342W

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-L340W

Plasmid

#160535

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-L340W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, L340W

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-N338W

Plasmid

#160534

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-N338W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, N338W

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-Y336W

Plasmid

#160533

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-Y336W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, Y336W

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB1lock-N342W

Plasmid

#160529

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-N342W

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, N342W

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB1lock-L340W

Plasmid

#160528

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-L340W

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, L340W

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB1lock-N338W

Plasmid

#160527

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-N338W

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, N338W

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB1lock-Y336W

Plasmid

#160526

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-Y336W

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, Y336W

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-prepore-N342C

Plasmid

#160521

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-prepore-N342C

Tags

His-Tag

Expression

Bacterial

Mutation

I104C, G244C, N342C

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-prepore-L340C

Plasmid

#160520

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-prepore-L340C

Tags

His-Tag

Expression

Bacterial

Mutation

I104C, G244C, L340C

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:S11-GFP1–9

Plasmid

#125665

Purpose

Binary vector for the inducible expression of S11-GFP1–9 fragment that can be used to detect a protein tagged with GFP10

Depositor

Tzu-Yin Liu

Publication

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

S11-GFP1–9

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-mHdac1-L2

Plasmid

#122307

Purpose

Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Hdac1 (Hdac1 Synthetic)

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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