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Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75244

Purpose

CRISPR/Cas9 plasmid against human Helios

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Helios (IKZF2 Human)

Use

CRISPR

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN4 + PGK-blast

Plasmid

#167825

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/Strep-HA-ZAKalpha_deltaS

Plasmid

#141197

Purpose

Expresses Strep-HA-ZAKalpha_deltaS in mammalian cells, can be used to make inducible cell lines

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 (MAP3K20 Human)

Tags

Strep, HA

Expression

Mammalian

Mutation

deletion of amino acids 670-712

Promoter

CMV

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-EHC

Plasmid

#112619

Purpose

expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-Emerald (H2BC3 Aequorea victoria, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Tags

Venus

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEHC

Plasmid

#112615

Purpose

promoterless expression plasmid driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-Emerald (H2BC3 Aequorea victoria, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Promoterless vector, ready for promoter for mamma…

Tags

Emerald

Expression

Mammalian

Promoter

No Promoter

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pmTHC

Plasmid

#112616

Purpose

promoterless expression plasmid driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-mTFP1 (H2BC3 Human, Anthozoa)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Promoterless vector, ready for promoter for mamma…

Tags

mTeal (mTFP1)

Expression

Mammalian

Promoter

No Promoter

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLL5.0 mCherry_K-RasC14

Plasmid

#101286

Purpose

Lentiviral expression of K-Ras C14

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

K-Ras (KRAS Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

C14

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75238

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75239

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-NRCAM 12950 (VL + VH) synNotch

Plasmid

#247509

Purpose

Expresses a synNotch receptor using a scFvs binding to NrCAM (neural cell adhesion molecule)

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

synNotch binding to NrCAM (neural cell adhesion molecule)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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PB-EF1-H4Δ1-19-HaloTag-IRES-Neo

Plasmid

#247451

Purpose

Expresses H4Δ1-19-Halo under EF1 promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4Δ1-19 lacks the initial 19 residues at the N-te…

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-NRCAM 12955 (VH+VL) synNotch

Plasmid

#247512

Purpose

Expresses a synNotch binding to NrCAM (neural cell adhesion molecule)

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch binding to NrCAM (neural cell adhesion molecule)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-NRCAM 12955 (VL + VH) synNotch

Plasmid

#247511

Purpose

Expresses a synNotch binding to NrCAM (neural cell adhesion molecule)

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch binding to NrCAM (neural cell adhesion molecule)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-SOX18-Brd 489

Plasmid

#219383

Purpose

Transcription factor SOX18 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

SOX18 (SOX18 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-SOX2-Brd 498

Plasmid

#219392

Purpose

Transcription factor SOX2 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

SOX2 (SOX2 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-KDM2B-Brd 478

Plasmid

#219378

Purpose

Transcription factor KDM2B with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

KDM2B (KDM2B Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-MYT1L-Brd 364

Plasmid

#219289

Purpose

Transcription factor MYT1L with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

MYT1L (MYT1L Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-NFKB2-Brd 359

Plasmid

#219285

Purpose

Transcription factor NFKB2 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

NFKB2 (NFKB2 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-MAX-Brd 346

Plasmid

#219272

Purpose

Transcription factor MAX with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

MAX (MAX Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-TEAD2-Brd 300

Plasmid

#219236

Purpose

Transcription factor TEAD2 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

TEAD2 (TEAD2 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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