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Plasmid#41562DepositorInsertUra3-FUS1-UBIYdkGFP*-SpHis5-Tim9
UseTagsExpressionMutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…PromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
FH95pUp95GW (B4)
Plasmid#74013Purposelentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNADepositorInsertPsd95 shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
IBMc233
Plasmid#161939PurposeCarries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoterDepositorInsertpSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
UseTagsExpressionBacterialMutationPromoterPPhlFAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSU314
Plasmid#169696PurposeTemplate plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xHA epitope tag adjacent to FRT-flanked cat (CamR) gene
UseTags1xHA tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSU313
Plasmid#169695PurposeTemplate plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
UseTags1xFLAG tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBP-pET-RBS
Plasmid#72981PurposePET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)DepositorInsertpET RBS derived from pET vectors
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNC1137
Plasmid#41563DepositorInsertUra3-FUS1-UBIMdkGFP*-SpHis5-Tim9
UseTagsExpressionMutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…PromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
FH95pUP95PDZGW (C6)
Plasmid#74022Purposelentiviral expression of Psd95 shRNA and Psd95-EGFP fusionDepositorInsertPsd95
UseLentiviral and RNAiTagsExpressionMammalianMutationPDZPromoterH1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP
Plasmid#72954PurposeLevel 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - A site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-scrCdh10.1 GFP
Plasmid#209102PurposeGFP expressing scramble of shRNA targeting Cdh10DepositorInsertscrCdh10.1
UseTagsExpressionMutationPromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shCdh20.1 GFP
Plasmid#209105PurposeGFP expressing shRNA targeting Cdh20DepositorInsertshCdh20.1
UseTagsExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR-MRE153-mut4
Plasmid#178909Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseTagsExpressionMutationTGC>CAT miR153-3p MRE mutatedPromoterSV40 promoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
IBMc050
Plasmid#161938PurposeCarries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoterDepositorInsertpSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
UseTagsExpressionBacterialMutationPromoterPlux2Available sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
FH95pUP95Q15AD45_64GW (D8)
Plasmid#74031Purposelentiviral expression of Psd95 shRNA and Psd95-Q15A _45_64-EGFP fusionDepositorInsertPsd95
UseLentiviral and RNAiTagsExpressionMammalianMutationQ15A, D45_64PromoterH1Available sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FH95pUP95L460pGW (E2)
Plasmid#74032Purposelentiviral expression of Psd95 shRNA and Psd95-L460P-EGFP fusionDepositorInsertPsd95
UseLentiviral and RNAiTagsExpressionMammalianMutationL460PPromoterH1Available sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FH95pUP95DSKIrGW (D2)
Plasmid#74025Purposelentiviral expression of Psd95 shRNA and Psd95dSK and EGFPDepositorInsertPsd95
UseLentiviral and RNAiTagsExpressionMammalianMutationdelta SKPromoterH1Available sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FH95pUG+p97W (B8)
Plasmid#74017Purposelentiviral expression of Psd95 shRNA, and EGFP-SAP97beta fusionDepositorInsertPsd95 shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-J23108
Plasmid#72964PurposeLevel 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)DepositorInsertJ23108 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-J23114
Plasmid#72965PurposeLevel 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)DepositorInsertJ23114 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7_RBS
Plasmid#72978PurposeT7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)DepositorInsertT7+RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only