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pEGFP-C1_N-NLS_L704A_F707A_F708A POLH

Plasmid

#221873

Purpose

Expresses PIP box-mutated POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Mutation

L704A_F707A_F708A

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT POLH

Plasmid

#221897

Purpose

Expresses WT POLH with a FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_D652A POLH

Plasmid

#222006

Purpose

Expresses D652A POLH with a FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH D652A. Coding sequence has been optimised fo…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_K682A POLH

Plasmid

#221862

Purpose

Expresses POLH mutated at K682A with a FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682A. Coding sequence has been optimised fo…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_K709A POLH

Plasmid

#221863

Purpose

Expresses POLH mutated at K709A with a FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K709A. Coding sequence has been optimised fo…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_POLH_ΔGG ubiquitin

Plasmid

#221869

Purpose

Expresses GFP-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP and ΔGG ubiquitin

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-mAnkrd11 (intron 4)

Plasmid

#236609

Purpose

Knockouts Ankrd11 in mouse cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

Ankrd11 (Ankrd11 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 6

Plasmid

#236623

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1831-2663

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 2

Plasmid

#236619

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1-1006

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-2)

Plasmid

#236613

Purpose

Knockouts ANKRD11 in human cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 1

Plasmid

#236618

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1-658

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-1)

Plasmid

#236612

Purpose

Knockouts ANKRD11 in human cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Use

CRISPR

Expression

Mammalian

Available Since

June 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-mAnkrd11 (intron 3)

Plasmid

#236608

Purpose

Knockouts Ankrd11 in mouse cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

Ankrd11 (Ankrd11 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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CIRC#del

Plasmid

#186344

Purpose

Contains VEGFA exon 7 and short intronic flanking regions not sufficient for expression of hsa_circ_0076611 (circVEGF)

Depositor

Giovanni Blandino

Article

Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

Insert

VEGFA (VEGFA Human)

Expression

Mammalian

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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CIRC

Plasmid

#186343

Purpose

Contains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)

Depositor

Giovanni Blandino

Article

Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

Insert

VEGFA (VEGFA Human)

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

Plasmid

#62580

Purpose

Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Mutation

miR-21 binding site 1 mutant (TAAGCTA --> TAGA…

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

Plasmid

#62581

Purpose

Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Mutation

miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
MSCV-human Erbb2-IRES-GFP

Plasmid

#91888

Purpose

Retroviral expression of human Erbb2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Erbb2 (ERBB2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

MMLV gag

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Ezh2 Y637S-IRES-GFP

Plasmid

#91887

Purpose

Retroviral expression of mouse Ezh2 with Y637S

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Y637S

Promoter

MMLV gag

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Ezh2 delta SETdomain-IRES-GFP

Plasmid

#91885

Purpose

Retroviral expression of mouse Ezh2 with a SET domain deletion

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

SET domain deleted

Promoter

MMLV gag

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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