Addgene

We narrowed to 1,130 results for: SAM-2

Showing: 1021 - 1040 of 1130 results

Results per page:

Sort results by:

pPOTv7-blast-lssmOrange

Plasmid

#179795

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

lss mOrange

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-blast-3Ty:mNG:3Ty

Plasmid

#179783

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLz54_polyPro_polar

Plasmid

#140847

Purpose

Same as pLz47 but with 20 polar amino acids instead of N*- peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with polar residues

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLz58_polyPro_CsoS2_CTP

Plasmid

#140850

Purpose

Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 C-terminal peptide

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLz55_polyPro_random_Npeptide

Plasmid

#140846

Purpose

Same as pLz47 but with randomized N*-peptide sequence

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with randomized CsoS2 N-peptide

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLz56_polyPro_CsoS2_MR_r1

Plasmid

#140848

Purpose

Same as pLz47 but with MR repeat 1 instead of N*-peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 MR repeat 1

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLz57_polyPro_CsoS2_CTD_r8

Plasmid

#140849

Purpose

Same as pLz47 but with CTD repeat 8 instead of N*-peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 CTD repeat 8

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBJT001

Plasmid

#225152

Purpose

High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

FLAG epitope tag (35 bp)

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBJT011

Plasmid

#225162

Purpose

Low copy cloning vector for integration of C-terminal FLAG epitope tag (GmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

FLAG epitope tag (35 bp)

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBJT007

Plasmid

#225158

Purpose

Low copy cloning vector for integration of C-terminal FLAG epitope tag (AmpR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

FLAG epitope tag (35 bp)

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBJT013

Plasmid

#225164

Purpose

Low copy cloning vector for integration of C-terminal FLAG epitope tag (SmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

FLAG epitope tag (35 bp)

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBJT009

Plasmid

#225160

Purpose

Low copy cloning vector for integration of C-terminal FLAG epitope tag (TetR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

FLAG epitope tag (35 bp)

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBJT005

Plasmid

#225156

Purpose

Low copy cloning vector for integration of C-terminal FLAG epitope tag (ChlR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

FLAG epitope tag (35 bp)

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR 223 VPS4B

Plasmid

#169320

Purpose

Gateway entry vector for human VPS4B ORF

Depositor

Francisca Vazquez

Article

Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

Insert

VPS4B (VPS4B Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

NA

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-ACE2 receptor 19-615

Plasmid

#167012

Purpose

Gateway-compatible Entry vector encoding ACE2 receptor (aa19-615) interacting with spike (S1) RBD domain from SARS-CoV-2

Depositor

Spyros Petrakis

Article

Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

Insert

ACE2 (ACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Amino acids 19-615, No stop codon

Promoter

Available since

March 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pYX-N-Puro-mNeon-TSC22D1

Plasmid

#222667

Purpose

endogenously tag TSC22D1 with N-terminal mNeon

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D1 (TSC22D1 Human)

Use

Tags

mNeon

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384)

Plasmid

#185488

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

Depositor

Benjamin Kleinstiver, Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

human codon usage NLS-anCas(BCA)-BPNLS-P2A-eGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS-P2A-eGFP and NLS

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)

Plasmid

#185485

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

Depositor

Benjamin Kleinstiver, Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

human codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS-P2A-eGFP and NLS

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

Plasmid

#185490

Purpose

CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

Depositor

Benjamin Kleinstiver, Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-eGFP

Expression

Mammalian

Mutation

H840A

Promoter

CMV and T7

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-NLS(SV40)-anCas(SCA)-BPNLS(SV40)-P2A-EGFP (LTH1378)

Plasmid

#185484

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

Depositor

Benjamin Kleinstiver, Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

human codon usage NLS-anCas(SCA)-BPNLS-P2A-eGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS-P2A-eGFP and NLS

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge