We narrowed to 8,996 results for: app

1. pJK289

   Plasmid

   #71553

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with Lys39>Ala mutant (AaAlr-K39A)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes lysine-39 to alanine (please see deposito…

   Promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK501

   Plasmid

   #72246

   Purpose

   Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   T. J. Kappock

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Gln206 to Arg and changes tryptophan-151 to pheny…

   Promoter

   T7 and endogenous

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK502

   Plasmid

   #71582

   Purpose

   Produces Acetobacter aceti 1023 putative SixA with C-terminal His6 tag (SixAH6)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

   Insert

   phosphohistidine phosphatase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK304

   Plasmid

   #72225

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala368>Asp mutant (H6AaAlr-A368D)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes alanine-368 to aspartate

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK303

   Plasmid

   #72224

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala367>Asp mutant (H6AaAlr-A367D)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes alanine-367 to aspartate

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK579

   Plasmid

   #72426

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6), additional unique internal restriction enzyme sites

   Depositor

   T. J. Kappock

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   4 silent mutations introduce unique internal SpeI…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK522

   Plasmid

   #72510

   Purpose

   Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK600

   Plasmid

   #71605

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-H59N,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-34 to phenylalanine, histidine…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGMEH-10M1-clpX-K127R

   Plasmid

   #27374

   Depositor

   Dirk Schnappinger

   Article

   Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

   Insert

   tetR(sB), clpXtb-K127R
   

   Use

   Gateway destination vector

   Tags

   Expression

   Bacterial

   Mutation

   changed Lysine 127 to Arginine

   Promoter

   Available since

   May 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJBL2191

    Plasmid

    #71205

    Purpose

    pbuE STAR Antisense Expression Plasmid

    Depositor

    Julius Lucks

    Article

    Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

    Insert

    pbuE STAR Antisense
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJBL1864

    Plasmid

    #71210

    Purpose

    Anti-anti Sense Expression Plasmid

    Depositor

    Julius Lucks

    Article

    Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

    Insert

    Anti-anti Sense
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJEC779

    Plasmid

    #234348

    Purpose

    Modular RENDR, GFP output, targets synthetic target

    Depositor

    James Chappell

    Article

    Gambill et al Nat Commun. 2023 Feb 1;14(1):543. doi: 1

    Insert

    Modular RENDR, GFP output
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PJ23119

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. LP580

    Plasmid

    #90205

    Purpose

    pCMV-myc with cDNA encoding HsTRAPPC13 full length

    Depositor

    Lotte Pedersen

    Article

    Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

    Insert

    TRAPPC13 (TRAPPC13 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    full length protein, no mutations

    Promoter

    CMV

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. LP539

    Plasmid

    #90203

    Purpose

    pCMV-myc with cDNA encoding HsTRAPPC8 aa residues 1-700

    Depositor

    Lotte Pedersen

    Article

    Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

    Insert

    TRAPPC8 (TRAPPC8 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    aa residues 1-700

    Promoter

    CMV

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK372

    Plasmid

    #73597

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-15 fatty acid desaturase
    

    Use

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK370

    Plasmid

    #73595

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 2)
    

    Use

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Mutation

    Silent mutation in Arg299 codon.

    Promoter

    GAL1 and GAL10

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK057

    Plasmid

    #75036

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase, N-terminal His6 (H6EcPurE1) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase (purK Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes residues 2-7 to His (SSRNNP>HHHHHH)

    Promoter

    T7 and n/a

    Available since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK350

    Plasmid

    #71560

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala365>Asp mutant (H6AaAlr-A365D)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    mutation changes alanine-365 to aspartate

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTIPI_mK_Anti-HA [12CA5]

    Plasmid

    #210657

    Purpose

    Mammalian expression of Anti-HA [12CA5] mouse kappa light chain; to be used with pTIPI_mIGg2a_Anti-HA [12CA5] heavy chain (Plasmid 210656) to make the antibody.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Anti-HA [12CA5] kappa light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Mouse kappa light chain

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pTIPI_mK-Anti_DYKDDDDK [M2]

    Plasmid

    #210655

    Purpose

    Mammalian expression of Anti-DYKDDDDK [M2] kappa light chain; to be used with pTIPI_mIGg2a_Anti-DYKDDDDK [M2] heavy chain (Plasmid 210654) to make the antibody.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Anti-DYKDDDDK [M2] kappa light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Mouse kappa light chain

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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