We narrowed to 23,671 results for: CRISPR

1. pRSFDuet1-sgRNA(siteA)

   Plasmid

   #154831

   Purpose

   sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site A in CHO cells

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Insert

   sgRNA(siteA)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRAU168

   Plasmid

   #86834

   Purpose

   bacterial expression of anti-CRISPR protein AcrIIA1

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA1
   

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDonor_AcCAST_kanR

   Plasmid

   #127925

   Purpose

   Kanamycin resistance donor for AcCAST

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   KanR
   

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJET1.2-STOP-dsRed

   Plasmid

   #60944

   Purpose

   Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.

   Depositor

   Frank Schnorrer

   Article

   Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

   Insert

   dsRED
   

   Use

   Unspecified

   Promoter

   3XP3

   Available Since

   Nov. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. hfDECOR-F148A

   Plasmid

   #219546

   Purpose

   Expression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

   Insert

   hfDECOR-F148A
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Changed TadA8e Phenylalanine 148 to Alanine

   Promoter

   CMV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. hfDECOR-V106G

   Plasmid

   #219547

   Purpose

   Expression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

   Insert

   hfDECOR-V106G
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Changed TadA8e Valine 106 to Glycine

   Promoter

   CMV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAS9-BFP empty

   Plasmid

   #80976

   Purpose

   Cas9, TagBFP, and sgRNA expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6xHis-MBP-huBoCas12a

   Plasmid

   #174673

   Purpose

   Expresses 6xHis-MBP tagged BoCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huBoCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAH01Sp::Cas9D10A

   Plasmid

   #128161

   Purpose

   Ptet-controlled expression of S. pyogenes Cas9D10A variant

   Depositor

   Calvin Henard

   Article

   Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

   Insert

   Sp Cas9
   

   Expression

   Bacterial

   Mutation

   D10A

   Promoter

   Ptet

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis-MBP-huPb2Cas12a

    Plasmid

    #174686

    Purpose

    Expresses 6xHis-MBP tagged Pb2Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPb2Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEntr_PE2

    Plasmid

    #149548

    Purpose

    Gateway entry clone with PE2 enzyme (with stop codon)

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    PE2
    

    Use

    CRISPR; Gateway shuttle vector

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. BCJJ125

    Plasmid

    #117515

    Purpose

    Cas9_3 (with intron) Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_3(intron):GCTT:BsaI
    

    Use

    CRISPR

    Tags

    2xFLAG and NLS

    Expression

    Plant

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSW21

    Plasmid

    #86836

    Purpose

    bacterial expression of anti-CRISPR protein AcrIIA4

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA4
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3xP3-EGFP.vas-int.NLS

    Plasmid

    #60948

    Purpose

    Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    vas-phiC31 integrase.NLS
    

    Use

    Rmce

    Promoter

    vasa

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pDD121

    Plasmid

    #91833

    Purpose

    Cas9 expression in C. elegans

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pECNUS_MP02

    Plasmid

    #194351

    Purpose

    CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

    Depositor

    Eunyoung Chae

    Article

    Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

    Insert

    pcoCas9
    

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMB1609_pRR-EGFP

    Plasmid

    #65852

    Purpose

    EGFP-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

    Depositor

    Marc Bühler

    Article

    Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

    Insert

    split EGFP coding sequence
    

    Use

    CRISPR and TALEN

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pgRNA-R-gfp

    Plasmid

    #220927

    Purpose

    Expresses gfp using J23119 promoter and pgRNA_AT backbone

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Insert

    superfolder GFP
    

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAS9-mCherry-TUBB

    Plasmid

    #66942

    Purpose

    CRISPaint target selector TUBB

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA TUBB
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSFDuet1-sgRNA(siteT9)

    Plasmid

    #154832

    Purpose

    sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site T9 in CHO cells

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Insert

    sgRNA(siteT9)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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