We narrowed to 23,671 results for: CRISPR

1. pCJH019_SC101_pLAC_Cas12c(4)_Kan

   Plasmid

   #183071

   Purpose

   Wild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c_4
   

   Expression

   Bacterial

   Mutation

   Wild-type

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMB1-A-pBAD-dCas9

   Plasmid

   #190132

   Purpose

   Level 1 MoClo plasmid containing an arabinose inducible dCas9 for CRISPRi

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   dCas9
   

   Use

   CRISPR

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFH66

   Plasmid

   #131765

   Purpose

   Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMB1-A-ptet-dCas9

   Plasmid

   #190129

   Purpose

   Level 1 MoClo plasmid containing an aTC inducible dCas9 for CRISPRi

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   dCas9
   

   Use

   CRISPR

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ACE2 g4 + Cas9

   Plasmid

   #153014

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + ACE2 gRNA (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   TagBFP2

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH54

   Plasmid

   #128183

   Purpose

   Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRAU171

   Plasmid

   #86835

   Purpose

   bacterial expression of anti-CRISPR protein AcrIIA2

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA2
   

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. p426*-LbSgH

   Plasmid

   #136992

   Purpose

   Helper plasmid for LbCpf1 gRNA cloning and expression

   Depositor

   Huimin Zhao

   Article

   Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAN-PA4-RFP

   Plasmid

   #62250

   Purpose

   reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   mRFP
   

   Expression

   Bacterial

   Promoter

   pA4

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCJH021_SC101_pLAC_Cas12c2_Kan

    Plasmid

    #183072

    Purpose

    Wild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c2
    

    Expression

    Bacterial

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMpGE010-gR082

    Plasmid

    #188553

    Purpose

    Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)

    Depositor

    Yutaka Kodama

    Article

    Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

    Insert

    gR082
    

    Use

    CRISPR; Entry vector

    Available Since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBSKΔB-TRE-dCas9-EGFP

    Plasmid

    #62347

    Purpose

    Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

    Insert

    dCas9-EGFP
    

    Use

    CRISPR

    Tags

    HA and SV40 NLS

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRA03

    Plasmid

    #103141

    Purpose

    Triple gRNA expression separated with 28nt Csy4 motif

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    gRNAs
    

    Expression

    Yeast

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cas9m2

    Plasmid

    #47314

    Purpose

    Cas9 D10A+H840A

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    Cas9m2
    

    Use

    CRISPR

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAN-PA2-RFP

    Plasmid

    #62248

    Purpose

    reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    mRFP
    

    Expression

    Bacterial

    Promoter

    pA2

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJEC625

    Plasmid

    #174368

    Purpose

    Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFH55

    Plasmid

    #125814

    Purpose

    Level0 nSpCas9-PmCDA, Arabidopsis codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    nSpCas9-PmCDA, Arabidopsis codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. BCJJ180

    Plasmid

    #117516

    Purpose

    Cas9_4 Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_4:GCTT:BsaI
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Plant

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. DmU6 3p3 GFP LgRNA

    Plasmid

    #182207

    Purpose

    For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Drosophila

    Depositor

    Craig Montell

    Article

    Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

    Insert

    3XP3-GFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFH45

    Plasmid

    #125810

    Purpose

    Level0 nSpCas9-ABE7.10 base changer, human codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    nSpCas9-ABE7.10 base changer, human codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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