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pcDNA5/FRT/TO HSPA8

Plasmid

#19460

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA8 (HSPA8 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pTP261

Plasmid

#104166

Purpose

Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)

Tags

14x Histidine tag and NEDD8 from Brachypodium dis…

Expression

Bacterial

Mutation

1x Cysteine located at bps 493-495 in full sequen…

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF37-2xCSTREP

Plasmid

#136198

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF37

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF37

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-E-cadherin promoter

Plasmid

#61798

Purpose

Luciferase reporter containing the mouse E-cadherin promoter

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

-178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
AAV-hSyn-SpdCas9-W3SL

Plasmid

#210708

Purpose

This Plasmid express hSyn promoter driven SpdCas9

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

S. Pyogenes dCas9

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

D10A/H840A

Promoter

hSyn

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9

Plasmid

#84242

Purpose

Expresses GA-inducible KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9
GID1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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C terminal Flag Mios pRK5

Plasmid

#46326

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Mios (MIOS Human)

Tags

Flag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,42a]

Plasmid

#49333

Purpose

Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)

Plasmid

#238181

Purpose

Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF5 (KLF5 Human, Synthetic)

Use

Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS DNAJB1

Plasmid

#19549

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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AAV-mPGK-eGFP (beta-globin intron)

Plasmid

#162513

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intron

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

mPGK

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 p37

Plasmid

#169015

Purpose

Expresses GST-p37 (human, codon optimized for expression in bacteria)

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

p37 (UBXN2B Human)

Tags

GST

Expression

Bacterial

Mutation

sequence was codon optimized for expression in E.…

Promoter

tac

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat-mCherry

Plasmid

#181841

Purpose

mCherry-tagged PKA catalytic subunit for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hAtg13

Plasmid

#22875

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

autophagy-related genes 13 (ATG13 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

Plasmid

#106895

Purpose

Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9-ABI
GID1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

Expression

Mammalian

Promoter

CAG and PGK

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSEMS-Halo7Tag-hFis

Plasmid

#111136

Purpose

hFis with selflabeling tag, e.g. for fluorescence microscopy

Depositor

Karin Busch

Article

Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

Insert

human fission factor1 (FIS1 Human)

Tags

Halo7-tag

Expression

Mammalian

Mutation

*

Promoter

CMV

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep72

Plasmid

#136819

Purpose

Mammalian expression of the centrosomal protein Cep72 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep72 (CEP72 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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p20StAD

Plasmid

#190268

Purpose

Constitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.

Depositor

Assaf Mosquna

Article

Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2

Type

Empty backbone

Use

Synthetic Biology; Yeast genome-integrating vector

Tags

GAL4-AD

Promoter

TDH3

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST47-MCU-GFP

Plasmid

#31732

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

MCU (MCU Human)

Tags

GFP

Expression

Mammalian

Mutation

*

Promoter

CMV

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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TK-GFP-Nluc-P2A-neo-TK

Plasmid

#134896

Purpose

Expresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Insert

TK-GFP-Nluc-P2A-Neo-TK

Use

Cryptosporidium expression

Promoter

Cryptosporidium actin and enolase

Available Since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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