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NAX_CAGGS_CAS9_m4_KRAB_EBFP2

Plasmid

#167873

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRVdG-4FLPo

Plasmid

#122050

Purpose

Genome plasmid for deletion-mutant rabies virus encoding Flpo recombinase

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

FLPo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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M49 GST-TEV-Chibby-C

Plasmid

#17197

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

Chibby (CBY1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 64-126

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pCIneo-EloC

Plasmid

#31982

Depositor

Joan Conaway

Article

Brower et al J Biol Chem. 1999 May 7;274(19):13629-36

Insert

Elongin C (ELOC Human)

Expression

Mammalian

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only
pRSET-spa-GCaMP6f

Plasmid

#67563

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Tags

HIS-tag

Expression

Bacterial

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pTYF-super-mfPENK-2.2k-Venus-WPRE

Plasmid

#156400

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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M47 MBP-TEV-Chibby

Plasmid

#17195

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

Chibby (CBY1 Human)

Tags

MBP

Expression

Bacterial

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pWP1_mFTCD

Plasmid

#102317

Purpose

expression of mouse cDNA of FTCD, refractory to the guides in plasmids 1,2

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Ftcd (Ftcd Mouse)

Use

Lentiviral

Promoter

EF1alpha

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST myc MOV10 D645N

Plasmid

#51720

Purpose

plasmid expresses myc-tagged MOV10 D645N

Depositor

Markus Landthaler

Article

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Insert

MOV10 D645N (MOV10 Human)

Tags

myc

Expression

Mammalian

Mutation

D645N

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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GST-PRMT9 L182A/D183A/I184A/G185A

Plasmid

#67608

Purpose

bacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

catalytic mutant LDIG(182-185)AAAA

Promoter

tac

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only
pPKm-243

Plasmid

#90506

Purpose

mOrange and mitochondrial-tagged sfGFP (pSIN-OFP-mitosfGFP)

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mOrange

Use

Lentiviral

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMT3-RhoGC

Plasmid

#100031

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC

Tags

1D4 T9'A (AETSQVAPA) and C8 (PRGPDRPEGIEE)

Expression

Mammalian

Promoter

Adenovirus major late promoter

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup88-mut

Plasmid

#87337

Purpose

To express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET27b-ecDHFR-N23PP/S148A

Plasmid

#110829

Purpose

Bacterial expression of E.coli DHFR N23PP/S148A mutant

Depositor

Stephen Benkovic

Article

Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

Insert

ecDHFR

Expression

Bacterial

Mutation

N23 mutated to P23; a proline residue inserted be…

Promoter

T7

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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D92_Ld_360170

Plasmid

#135905

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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p224 pCMV-RFP/CREM

Plasmid

#8401

Purpose

Floxed RFP inserted within the coding sequence of cre recombinase.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

RFP/CREM

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre, LOX 511 sites surrounding RFP withi…

Promoter

CMV

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pM117: VEGFA array presgRNA

Plasmid

#166869

Purpose

U6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Insert

Human VEGFA targeting array presgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCR4_SNORD27 probe

Plasmid

#73069

Purpose

antisense probe for detection of human SNORD27

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (SNORD2 Human)

Use

In vitro transcription

Promoter

t7

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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JB96

Plasmid

#128923

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

nRasSN-BirA*-HA-MAO (NRAS Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR V2 sgShmt2_2

Plasmid

#106315

Purpose

Express Cas9 and sgRNA targeting mShmt2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting mShmt2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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