We narrowed to 11,048 results for: ENA;

1. Perox-mTurq2-Apollo-NADP+

   Plasmid

   #193304

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Use

   Tags

   mTurqoise2, peroxisomal targeting sequence

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSynapsin- soCoChR-GFP

   Plasmid

   #107708

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Has Service

   AAV9

   Insert

   soCoChR-GFP
   

   Use

   AAV

   Tags

   EGFP and KA2

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ-CBE-SpRYn-Act3.0

   Plasmid

   #178958

   Purpose

   It consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   hA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPBpuro-EGFR(M721)

   Plasmid

   #197379

   Purpose

   Encoding human ErbB1 mutant.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   EGFR mutant (EGFR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lysine 721 to Methionine

   Promoter

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFR D837A

   Plasmid

   #11014

   Purpose

   Retroviral construct for expressing human EGFR with D837A mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR D837A (EGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   D837A

   Promoter

   Available Since

   Dec. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pYPQ-ABE-SpRYn-Act3.0

   Plasmid

   #178959

   Purpose

   It consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   ABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-EF1alpha-soCoChR-tdTomato

   Plasmid

   #107714

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Insert

   soCoChR-tdTomato
   

   Use

   AAV

   Tags

   KA2 and tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mito-mTurq2-Apollo-NADP+

   Plasmid

   #193303

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the mitochondria

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Use

   Tags

   mTurqoise2 and mitochondrial targeting sequence

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-neo-TET

   Plasmid

   #51286

   Purpose

   expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   U6 (RNU6-1 Human)
   

   Use

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Mutation

   Promoter

   U6 upstream enhancer sequence

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe EGFR(L861Q)

    Plasmid

    #32068

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    L861Q

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

11. lentiCRISPR v2-sgDHODH-4

    Plasmid

    #186022

    Purpose

    knock out DHODH in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EGFR-ET1

    Plasmid

    #44185

    Depositor

    Timothy Springer

    Article

    Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

    Insert

    EGFR-ET1 (EGFR Human)
    

    Use

    Tags

    3C cleavge site, protein C peptide tag, his6, SBP…

    Expression

    Mammalian

    Mutation

    I104V and D254N relative to NP_005219.2

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-MTS-WT-c-Src-FLAG

    Plasmid

    #44652

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Use

    Tags

    FLAG tag and mitochondria-targeting sequence (res…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti hSyn GAPDH

    Plasmid

    #220918

    Purpose

    Neuronal specific expression of GAPDH

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    GAPDH (GAPDH Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF1a_FL MUT TET1

    Plasmid

    #124081

    Purpose

    Expression of catalytic inactive form of full-length TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 - full length, mutated (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    H1672Y, D1674A

    Promoter

    EF1 alpha

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Syn1-DIO-VGLUT2:miniSOG

    Plasmid

    #214200

    Purpose

    For Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)

    Depositor

    Thomas Hnasko

    Article

    Steinkellner et al ACS Chem Neurosci. 2021 Feb 17;12(4):626

    Insert

    Slc17a6 tagged with miniSOG and hemagglutinin
    

    Use

    AAV and Cre/Lox

    Tags

    miniSOG (tagged with HA) is fused to the first in…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIYN3

    Plasmid

    #197099

    Purpose

    To express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Biochem Biophys Res Commun. 2011 Aug 12;

    Insert

    TyrRS variant, Nap3 tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ala70, Thr158, Ser159, Arg286 (TyrRS)

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPR v2-sgDHODH-2

    Plasmid

    #186023

    Purpose

    knock out DHODH in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV hSyn1 YFP-CaRhAC

    Plasmid

    #101721

    Purpose

    Yellow fluorescent protein (YFP) – tagged rhodopsin adenylyl cyclase created from the rhodopsin guanylyl cyclase of Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellu

    Depositor

    Thomas Oertner

    Article

    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

    Insert

    YFP CaRhAC
    

    Use

    AAV

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1a_CD MUT TET1

    Plasmid

    #124083

    Purpose

    Expression of mutated catalytic domain only form of TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 - catalytic domain mutated (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    H1672Y, D1674A

    Promoter

    EF1 alpha

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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