We narrowed to 15,988 results for: LEA

1. pGBW-m4046228

   Plasmid

   #145400

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF7a protein (ORF7a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pEPQD0CM0281

   Plasmid

   #162302

   Purpose

   Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   N-terminal tag, [S-tag]-[TEV cleavage site]
   

   Use

   Synthetic Biology

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pREXNH3

   Plasmid

   #47079

   Purpose

   FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and N-terminal 10x His tag and 3C protease cleavage site

   Depositor

   Eric Geertsma, Raimund Dutzler

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Lactococcus lactis expression

   Tags

   3C protease site (PreScission site) and decaHis (…

   Promoter

   NIS (nisin)

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBW-m4046299

   Plasmid

   #153878

   Purpose

   Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pJA54

   Plasmid

   #60212

   Purpose

   cleavage near e1350 locus in sqt-1

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   sqt-1(e1350) gRNA1
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. GLYR1

   Plasmid

   #38946

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GLYR1 (GLYR1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. library backbone 3'LTR gRNA and barcode

   Plasmid

   #127397

   Purpose

   Digestion by BstXI and BamHI to release the backbone

   Depositor

   Xiaowei Zhuang

   Article

   Wang et al Proc Natl Acad Sci U S A. 2019 May 28;11

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Puro-mCherry

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBW-m4134207

   Plasmid

   #152431

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;3xFlag

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pMTB-Multibow-hB

   Plasmid

   #60992

   Purpose

   Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear EBFP2.

   Depositor

   Sean Megason

   Article

   Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

   Insert

   EBFP2
   

   Use

   Zebrafish

   Tags

   histone 2B tag

   Promoter

   Bactin2

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDXNSM3

    Plasmid

    #49028

    Purpose

    FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag and 3C protease cleavage site

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), MYC, and str…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGBW-m4133141

    Plasmid

    #152404

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    June 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pJA55

    Plasmid

    #59935

    Purpose

    gRNA for cleavage near sqt-1(e1350) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    sqt-1(e1350) gRNA2
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGBW-m4046457

    Plasmid

    #145424

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGBW-m4046390

    Plasmid

    #148996

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. 4YZ8

    Plasmid

    #69955

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WHSC1L1:JMC055-F10:C223308 (NSD3 Human)
    

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pSaI14

    Plasmid

    #8614

    Depositor

    Robert Hartley

    Article

    Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

    Insert

    Ribonuclease inhibitor SaI14
    

    Expression

    Bacterial

    Mutation

    N terminal threonine changed to alanine

    Available Since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pGBW-m4046207

    Plasmid

    #145559

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4046418

    Plasmid

    #149029

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046476

    Plasmid

    #145512

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pMTB-Multibow-hY

    Plasmid

    #60995

    Purpose

    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear citrine

    Depositor

    Sean Megason

    Article

    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

    Insert

    citrine
    

    Use

    Zebrafish

    Tags

    histone 2B tag

    Promoter

    Bactin2

    Available Since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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