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pMV-Tc1-1_PM w/o spacer

Plasmid

#218296

Purpose

Donor plasmid of Tc1-1_PM Transposon without spacer

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tc1-1_PM Transposon

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tc1-2_ST w/o spacer

Plasmid

#218297

Purpose

Donor plasmid of Tc1-2_ST Transposon without spacer

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tc1-2_ST Transposon

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.CBX8ΔChromo

Plasmid

#224708

Purpose

Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 chromodomain truncation (CBX8 Human)

Tags

HMBP

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.mEGFP-CBX8

Plasmid

#224710

Purpose

Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 (CBX8 Human)

Tags

mEGFP

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFB1.HMBP.PrS.CBX8-KR21A

Plasmid

#224709

Purpose

Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 KR21A mt (CBX8 Human)

Tags

HMBP

Expression

Insect

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.CBX8

Plasmid

#224707

Purpose

Expresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 (CBX8 Human)

Tags

HMBP

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7185

Plasmid

#219765

Purpose

Vector for expression PzPKS2 in yeast (P.pastoris)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PzPKS2

Expression

Yeast

Promoter

pGAP

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7190

Plasmid

#219763

Purpose

MoClo-compatible Level M for expression nnLuz, nnH3H, nnCPH in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

nnLuz, nnH3H, nnCPH

Expression

Plant

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7186

Plasmid

#219758

Purpose

Vector for expression PpASCL in mammalian cells (HEK293NT)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PpASCL

Expression

Mammalian

Promoter

pCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7179

Plasmid

#219757

Purpose

Vector for expression HmS in mammalian cells (HEK293NT)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

HmS

Expression

Mammalian

Promoter

pCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 7 deletion

Plasmid

#224388

Purpose

Lenti plasmid for generating GNB1L _WD 7 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 7 domain deleted (aa 286-323)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion

Plasmid

#224387

Purpose

Lenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 6 domain deleted (aa 242-282)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletion

Plasmid

#224385

Purpose

Lenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 4 domain deleted (aa 153-195)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pX459_GNB1L_gRNA

Plasmid

#224377

Purpose

Targets GNB1L for dTAG C terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hAT-4_Crp

Plasmid

#217168

Purpose

Mammalian expression vector for expression of hAT-4_Crp transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

hAT-4_Crp transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-OposCharlie2

Plasmid

#217169

Purpose

Mammalian expression vector for expression of OposCharlie2 transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

OposCharlie2 transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hAT-1_AMi

Plasmid

#217170

Purpose

Mammalian expression vector for expression of hAT-1_Ami transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

hAT-1_AMi transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Mariner-2_AMi

Plasmid

#217171

Purpose

Mammalian expression vector for expression of Mariner-2_Ami transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Mariner-2_AMi transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hAT-13_AMi

Plasmid

#217172

Purpose

Mammalian expression vector for expression of hAT-13_Ami transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

hAT-13_AMi transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hAT-8_PM

Plasmid

#217149

Purpose

Mammalian expression vector for expression of hAT-8_PM transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

hAT-8_PM transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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