We narrowed to 12,237 results for: nsf

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pX459-HypaCas9-mR2-ECT2_sgRNA

Plasmid

#183875

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ECT2 sgRNA spacer (ECT2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHcRed-NPM1cdN243-C1

Plasmid

#131838

Purpose

Expresses the last 50 amino acids of HcRed-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Tags

HcRed

Expression

Mammalian

Mutation

This is a deletion contruct of the cytoplasmic m…

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHcRed-NPMMLF1nes-C1

Plasmid

#131849

Purpose

Expresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfection

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Tags

HcRed

Expression

Mammalian

Mutation

This is a control deletion contruct of the cytop…

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRB131

Plasmid

#180174

Purpose

pAAV construct GluN1A714L (shRNA resistant)

Depositor

Richard Bergeron

Article

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN1 (Grin1 Rat)

Use

AAV

Tags

Phluorin

Expression

Mammalian

Mutation

A714L

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iDianiSnFR_PM

Plasmid

#177759

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iDianiSnFR_PM

Use

AAV

Promoter

hSyn

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iDianiSnFR_ER

Plasmid

#177758

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iDianiSnFR_ER

Use

AAV

Promoter

hSyn

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iCyt_F_SnFR_PM

Plasmid

#177755

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iCyt_F_SnFR_PM

Use

AAV

Promoter

hSyn

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iCytSnFR_PM

Plasmid

#177753

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iCytSnFR_PM

Use

AAV

Promoter

hSyn

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iCytSnFR_ER

Plasmid

#177752

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iCytSnFR_ER

Use

AAV

Promoter

hSyn

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn iCyt_BrEt_SnFR_ER

Plasmid

#177756

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn iCyt_BrEt_SnFR_ER

Use

AAV

Promoter

hSyn

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iCyt_F_SnFR_ER

Plasmid

#177754

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iCyt_F_SnFR_ER

Use

AAV

Promoter

hSyn

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC1535 pscAAV mU6 shRNA(scram) CMV-IE Nuc-EYFP

Plasmid

#135563

Purpose

An AAV vector expressing scrambled shRNA and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

shRNA (scrambled)
Nuc-EYFP

Use

AAV and RNAi

Tags

3xNLS

Expression

Mammalian

Promoter

CMV-IE and mU6

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_Donor/Reporter-COL4A3

Plasmid

#130279

Purpose

COL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor template

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

mCherry-eGFP

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_COL4A3_self-cleavingCas9

Plasmid

#130281

Purpose

Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivation

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

spCas9

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_COL4A5_self-cleavingCas9

Plasmid

#130282

Purpose

Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivation

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

spCas9

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine

Plasmid

#160069

Purpose

Conditionally overexpress collybistin (CB2SH3-) and mCitrine

Depositor

Angel de Blas

Article

George et al eNeuro. 2021 Jun 2. pii: ENEURO.0561-20.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

AAV

Tags

HA- in CB2SH3

Promoter

human Synapsin promoter

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG

Plasmid

#154778

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

LaG17-SynNotch

Tags

LaG17

Expression

Mammalian

Mutation

204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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p-AAV2-hSyn-MerMAID1-Citrine

Plasmid

#126520

Purpose

Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

Insert

MerMAID1

Use

AAV

Tags

Citrine

Promoter

human synapsin

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-N entry vector

Plasmid

#111751

Purpose

Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Type

Empty backbone

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL405 CHYRON1 integration construct

Plasmid

#126442

Purpose

To integrate the CHYRON1 locus at HEK293site3.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6-CHYRON1 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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