We narrowed to 16,204 results for: grna

1. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pALPS puro miR30-L1221

   Plasmid

   #101335

   Purpose

   negative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGT

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   shRNA targeting miR30
   

   Use

   Lentiviral

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. px459 VRER

   Plasmid

   #101716

   Purpose

   sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E and T1337R)

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSuper-gmiR-124

   Plasmid

   #171555

   Purpose

   a human miR-124-expressing plasmid

   Depositor

   Yan Zeng

   Article

   Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

   Insert

   miR-124 (MIR124-1 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCCM935

   Plasmid

   #58202

   Purpose

   Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.

   Depositor

   Craig Mello

   Article

   Kim et al Genetics. 2014 May 30. pii: genetics.114

   Insert

   unc-22 sgRNA
   

   Expression

   Worm

   Promoter

   U6

   Available Since

   July 30, 2014

   Availability

   Academic Institutions and Nonprofits only

6. GFP-PICK

   Plasmid

   #31613

   Depositor

   Robert Malenka

   Article

   Citri et al J Neurosci. 2010 Dec 8. 30(49):16437-52.

   Insert

   PICK1 (shRNA insensitive) +shRNA towards PICK1 (Pick1 Rat)
   

   Use

   Lentiviral

   Tags

   GFP, HA, and PICK1 shRNA

   Expression

   Mammalian

   Available Since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pADH137

   Plasmid

   #90986

   Purpose

   NAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Inserts

   C. albicans LEU2 1 of 2
   pENO1
   Cas9
   NAT 1 of 2
   

   Use

   CRISPR

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiX2-PURO-shGFP

   Plasmid

   #61230

   Purpose

   Lentivirus expressing shRNA to GFP

   Depositor

   Reuben Shaw

   Article

   Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Promoter

   H1/TO

   Available Since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA6.2-GW/EmGFP-miR-SUMO23

   Plasmid

   #31073

   Depositor

   Wulf Paschen

   Article

   Yang et al Biochem Biophys Res Commun. 2009 Apr 24.

   Insert

   SUMO2/3 microRNA (SUMO2, SUMO3 Human)
   

   Use

   RNAi

   Tags

   EmGFP

   Expression

   Mammalian

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. dCas9v2

    Plasmid

    #103140

    Purpose

    TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    dCas9-VPR
    

    Expression

    Yeast

    Mutation

    Removed scaffold gRNA from original plasmid

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330A_D10A-1x7

    Plasmid

    #58777

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pADH140

    Plasmid

    #90988

    Purpose

    NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    C. maltosa LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. GEARBOCS-SPARCL1-N-HATag

    Plasmid

    #218185

    Purpose

    To tag Hevin with HA at the N-terminal

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. GEARBOCS-SPARCL1 KO

    Plasmid

    #218182

    Purpose

    To KO Hevin in astrocytes

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. GEARBOCS-SPARCL1-C-mCherryTag

    Plasmid

    #218183

    Purpose

    To tag Hevin with mCherry at its C-terminal

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSUPER.PKD3.RNAi

    Plasmid

    #10818

    Depositor

    Alex Toker

    Article

    Hausser et al Nat Cell Biol. 2005 Sep . 7(9):880-6.

    Insert

    PKD3 RNAi (PRKD3 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Oct. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pSBY2_ligD(FnCpf1)

    Plasmid

    #104623

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligD

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    crRNA to ligD
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Pj23119

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRetrosuper USP28 shRNA-3

    Plasmid

    #15664

    Depositor

    Martin Eilers

    Article

    Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

    Insert

    USP28 shRNA (USP28 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pLKO-shTRAF2#1

    Plasmid

    #44127

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

20. TET-pLKO.1 PURO shLuc

    Plasmid

    #83092

    Purpose

    Lentiviral shRNA vector for inducible knockdown of luciferase (control hairpin)

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shLuc
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only