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pEPMS0CM0033

Plasmid

#227512

Purpose

L0 part - CDS (without STOP codon)

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

CcTXSS (Cynara cardunculus taraxasterol synthase)

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEPMS0CM0036

Plasmid

#227513

Purpose

L0 part - CDS (without STOP codon)

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

HaTXSS (Helianthus annuus taraxasterol synthase)

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB027

Plasmid

#234636

Purpose

pET-28a(+) based plasmid for expression of H16_B1102 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1102 (H16_RS24270 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB028

Plasmid

#234637

Purpose

pET-28a(+) based plasmid for expression of H16_B1148 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1148 (H16_RS24495 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB029

Plasmid

#234638

Purpose

pET-28a(+) based plasmid for expression of H16_B1264 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1264 (H16_RS25065 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB030

Plasmid

#234639

Purpose

pET-28a(+) based plasmid for expression of H16_B1335 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1335 (H16_RS25400 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB024

Plasmid

#234633

Purpose

pET-28a(+) based plasmid for expression of H16_A0285 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A0285 (H16_RS01400 Cupriavidus necator H16)

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB026

Plasmid

#234635

Purpose

pET-28a(+) based plasmid for expression of H16_A3514 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A3514 (H16_RS17530 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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P-Bf-fusA2

Plasmid

#235658

Purpose

Reports Cur activity in Bacteroides fragilis

Depositor

Guy Townsend

Article

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides fragilis fusA2 promoter

Mutation

The B. thetaiotaomicron fusA2 promoter lacking &q…

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG014

Plasmid

#231335

Purpose

BEVA Golden Gate cloning vector; Narrow host range plasmid. L1-pMB1-Gm-ELT3; pNDGG021, pOGG009, pNDGG007, pOGG013. GmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pQGG003

Plasmid

#231330

Purpose

BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid with I-SceI counterselectable site for BpiI cloning; KmR/NmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pHW622

Plasmid

#229868

Purpose

Entry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)

Depositor

Han Wang

Article

Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

Insert

reverse FRT3 (rFRT3)

Expression

Worm

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-BASU-His

Plasmid

#218451

Purpose

E coli expression vector for Halo-BASU-His biotin ligase

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

HaloTag-BASU-His fusion protein

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pJUB1

Plasmid

#208813

Purpose

Expresses arabinose-inducible JUB1-derived artificial transcription factor in bacterial cell

Depositor

Marc Erhardt

Article

Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

Insert

JUB1 TF

Use

Synthetic Biology

Expression

Bacterial

Promoter

Arabinsoe-inducible promoter

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMK454 (dTAG-BSD)

Plasmid

#214384

Purpose

dTAG for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

dTAG-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSN993

Plasmid

#219961

Purpose

Expresses CMT2A mutant version of human KIF1Bß in C. elegans

Depositor

Shinsuke Niwa

Insert

unc-104 promoter::human KIF1Bß(Q98L) (KIF1B Human, Nematode)

Expression

Worm

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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p4P1R_EC1.2en-EC1.1p

Plasmid

#213912

Purpose

Entry clone containing Egg cell-specific promoter (EC1.1p) fused to EC1.2 enhancer (EC1.2en). For use in plants and compatible with the MultiSite Gateway system

Depositor

Sébastien Mongrand

Article

Li et al Bio Protoc. 2024 Jul 5;14(13):e5029. doi

Insert

EC1.2en-EC1.1p

Expression

Plant

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ223

Plasmid

#217497

Purpose

Bacterial expression of BaFnuc guide RNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

BaFnuc guide RNA scaffold

Expression

Bacterial

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ224

Plasmid

#217496

Purpose

Bacterial expression of BaFnuc for IVTT

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

BaFnuc

Expression

Bacterial

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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SNAPf-BASU-His

Plasmid

#218450

Purpose

E coli expression vector for SNAPf-BASU-His biotin ligase

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

SNAPf-BASU-His fusion protein

Expression

Bacterial

Promoter

T7

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGW1-ExSYTE-P2A-EGFP

Plasmid

#216257

Purpose

ExSYTE (P2A) EGFP

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE P2A EGFP

Expression

Mammalian

Available Since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGW1-ExSYTE-EGFP

Plasmid

#216259

Purpose

ExSYTE - EGFP fusion

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE EGFP

Expression

Mammalian

Available Since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGEM-HE-ExSYTE

Plasmid

#216265

Purpose

ExSYTE in oocyte vector

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE

Use

Crna synthesis for xenopus laevis oocyte

Tags

none

Available Since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTST-EGFP-GPIBY55

Plasmid

#213706

Purpose

Express the affinity sorting tag TST-EGFP- GPIBY55.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTST-EGFP-GPICEAM7

Plasmid

#213705

Purpose

Express the affinity sorting tag TST-EGFP- GPICEAM7.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTST-EGFP-GPIDAF

Plasmid

#213704

Purpose

Express the affinity sorting tag TST-EGFP- GPIDAF.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-TREER

Plasmid

#208321

Purpose

Bacterial expression of the His-tagged peptide TREER.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PuRRRE

Plasmid

#208327

Purpose

C-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PuRRRE

Tags

PuRRRE

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-TREER

Plasmid

#208325

Purpose

C-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TREER

Tags

TREER

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-PuRRRE

Plasmid

#208323

Purpose

Bacterial expression of the His-tagged peptide PuRRRE.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

PuRRRE

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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