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pET-47b-N-His-Ub2-I44AI120A

Plasmid

#253395

Purpose

Expression in E. coli of linear di-ubiquitin carrying the I44AI120A double mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Ubiquitin (RPS27A Human)

Tags

His

Expression

Bacterial

Mutation

I44AI120A

Availability

Academic Institutions and Nonprofits only

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pET-24-hexaUb-I44AI120A

Plasmid

#253397

Purpose

Expression in E. coli of linear hexa-ubiquitin carrying the I44AI120A mutation, with His tag at C-terminus.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Ubiquitin (RPS27A Human)

Tags

His

Expression

Bacterial

Mutation

I44AI120A

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-SPRTN-deltaC (Lys241AsnfsX8)

Plasmid

#253399

Purpose

Expression in E. coli of SPRTN-deltaC patient variant (Lys241AsnfsX8).

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

SPRTN (SPRTN Human)

Tags

GST

Expression

Bacterial

Mutation

Lys241AsnfsX8

Availability

Academic Institutions and Nonprofits only

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pNIC28-SPRTN-UBZ (452-489), C456AC459A

Plasmid

#253401

Purpose

Expression in E. coli of SPRTN-UBZ domain carrying the C456AC459A double mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

SPRTN (SPRTN Human)

Tags

His

Expression

Bacterial

Mutation

C456AC459A

Availability

Academic Institutions and Nonprofits only

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pNIC28-SPRTN core (26-240)-E112Q

Plasmid

#253403

Purpose

Expression in E. coli of SPRTN 26-240 truncation, carrying the E112Q mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

SPRTN (SPRTN Human)

Tags

His

Expression

Bacterial

Mutation

E112Q

Availability

Academic Institutions and Nonprofits only

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pNIC28-SPRTN core (26-240)-L38A

Plasmid

#253404

Purpose

Expression in E. coli of SPRTN 26-240 truncation, carrying the L38A mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

SPRTN (SPRTN Human)

Tags

His

Expression

Bacterial

Mutation

L38A

Availability

Academic Institutions and Nonprofits only

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pNIC28-SPRTN core (26-240)-L99A

Plasmid

#253405

Purpose

Expression in E. coli of SPRTN 26-240 truncation, carrying the L99A mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

SPRTN (SPRTN Human)

Tags

His

Expression

Bacterial

Mutation

L99A

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-SPRTN-ZBD-BR (151-245)

Plasmid

#253406

Purpose

Expression in E. coli of SPRTN-ZBD-BR truncation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

SPRTN (SPRTN Human)

Tags

GST

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pCold I-SPRTN-PIP-UBZ (320-489)

Plasmid

#253407

Purpose

Expression in E. coli of SPRTN-PIP-UBZ truncation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

SPRTN (SPRTN Human)

Tags

His

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#194703

Purpose

Inducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

RPS2 (RPS2 Human)

Use

Lentiviral

Tags

APOBEC1-HA-P2A-mRuby

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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