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pIRES2-eGFP:HA-mTREK-1(S131C/A286F)

Plasmid

#224775

Purpose

Mammalian expression vector for generating mouse TREK-1

Depositor

Dan Minor

Publication

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Expression

Mammalian

Mutation

S131C / A286F

Promoter

CMV

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTRAAK(S93C/Q233K)

Plasmid

#224768

Purpose

In vitro transcription for Oocyte expression of mTRAAK(S93C/Q233K)

Depositor

Dan Minor

Publication

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (Kcnk4 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S93C / Q233K

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (S131A/C159A/C219A)

Plasmid

#224753

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A)

Depositor

Dan Minor

Publication

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131A/C159A/C219A

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (S131C/C159A/C219A)

Plasmid

#224752

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A)

Depositor

Dan Minor

Publication

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C/C159A/C219A

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.SLC17A1-flag

Plasmid

#224418

Purpose

Express SLC17A1 in mammalian cells

Depositor

Jonathan Long

Insert

Solute carrier family 17 (sodium phosphate), member 1 (SLC17A1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28-mTFP1

Plasmid

#223638

Purpose

Lentiviral expression of human CD28 with mTFP1 tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

mTFP1

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28(Y209F)

Plasmid

#223627

Purpose

Lentiviral expression of human CD28 (Y209F) in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Mutation

Y209F

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28(Y191F)

Plasmid

#223625

Purpose

Lentiviral expression of human CD28 (Y191F) in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Mutation

Y191F

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28-LgBiT

Plasmid

#223616

Purpose

Lentiviral expression of human CD28 with LgBiT tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

LgBiT

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28-CLIP

Plasmid

#223610

Purpose

Lentiviral expression of human CD28 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28

Plasmid

#223600

Purpose

Lentiviral expression of human CD28 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-39S Light chain

Plasmid

#216297

Purpose

Expresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S Fab

Depositor

Mark Howarth

Publication

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

39S Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MF3958 Light chain

Plasmid

#216304

Purpose

Expresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 Fab

Depositor

Mark Howarth

Publication

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

MF3958 Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKL2-160

Plasmid

#219414

Purpose

Estradiol-inducible expression of 13-6 Zinc finger activator

Depositor

Ahmad Khalil

Publication

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-WT)-PDZLigWT-tSSA1}

Expression

Yeast

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast CG1358

Plasmid

#218541

Purpose

Drosophila CG1358 cDNA (human codon optimized)

Depositor

Kivanc Birsoy

Publication

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CG1358 (HisT Fly)

Use

Retroviral

Expression

Mammalian

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro SPNS1_sg2

Plasmid

#218531

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Publication

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro SPNS1_sg1

Plasmid

#218530

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Publication

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v1 GFP FLVCR1_sg5

Plasmid

#218521

Purpose

sgRNA targeting human FLVCR1

Depositor

Kivanc Birsoy

Publication

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

CRISPR and Lentiviral

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pKH160_FLAG-APEX2-YIPF4 LIR mut

Plasmid

#214979

Purpose

Flag-APEX2 tagged LIR mutant YIPF4

Depositor

Wade Harper

Publication

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2_YIPF4_mut (YIPF4 Human)

Use

Lentiviral

Mutation

LIR mutant

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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TR knockout HRD

Plasmid

#207579

Purpose

Homologous recombination donor replacing TR with a puro cassette.

Depositor

Jens Schmidt

Publication

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

puro resistance cassette flanked by TR locus sequences excluding TR

Tags

None

Expression

Mammalian

Promoter

SV40

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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