We narrowed to 16,204 results for: grna

1. pDrop

   Plasmid

   #184872

   Purpose

   Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pYPQ132D

   Plasmid

   #69288

   Purpose

   Golden Gate entry vector; 1st gRNA under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLKO.1P shNFS1_1

   Plasmid

   #102963

   Purpose

   Suppression of NFS1

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shNFS1_1 (NFS1 Human)
   

   Use

   Lentiviral and RNAi

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. px330 VRER

   Plasmid

   #101729

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLKO.1 RagB shRNA #2

   Plasmid

   #26628

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RagB shRNA (RRAGB Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pSuper-gmiR-1

   Plasmid

   #171553

   Purpose

   a human miR-1-expressing plasmid

   Depositor

   Yan Zeng

   Article

   Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

   Insert

   miR-1-2 (MIR1-2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYPQ131D

   Plasmid

   #69287

   Purpose

   Golden Gate entry vector; 1st gRNA under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiCRISPRv2 sgSp1

   Plasmid

   #174907

   Purpose

   Second generation sgRNA against Sp1

   Depositor

   Jane Clifford

   Article

   Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

   Insert

   Specificity Protein 1 (SP1 Human)
   

   Expression

   Mammalian

   Mutation

   Missense mutation to mutate PAM sequence

   Promoter

   EFS-NS

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CAG-mCherry-Otx2shRNA1

   Plasmid

   #73979

   Purpose

   Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the mouse Otx2 gene
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO-shTRAF2#2

    Plasmid

    #44128

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pDYSCKO-ADE1

    Plasmid

    #120264

    Purpose

    pDYSCKO canavanine co-selection vector with a guide targeting the ADE1 locus

    Depositor

    Christian Landry

    Article

    Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

    Insert

    pDYSCKO cassette-ADE1 targeting guide
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    SNR52

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330A_FokI-1x2

    Plasmid

    #63602

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMKO.1 GFP B56 gamma3 shRNA 14-1

    Plasmid

    #10681

    Depositor

    William Hahn

    Article

    Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

    Insert

    PP2A B56 gamma3 shRNA (PPP2R5C Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pBrain-GFP-shGL2

    Plasmid

    #60004

    Purpose

    Co-expresses control GL2 shRNA and GFP.

    Depositor

    Stephen Royle

    Article

    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

    Insert

    EGFP
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p330 VQR

    Plasmid

    #101730

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS423-TyrSgH

    Plasmid

    #163973

    Purpose

    Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TET-pLKO.1 PURO shGFP

    Plasmid

    #83085

    Purpose

    Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin)

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

19. pMKO.1-Puro-shPP2A-Abeta

    Plasmid

    #15250

    Depositor

    William Hahn

    Article

    Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

    Insert

    PP2A regulatory subunit A, beta isoform (PPP2R1B Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pLentiCRISPRv2-sgLmnb2-HepmCherry

    Plasmid

    #192830

    Purpose

    Expresses sgRNA targeting Lmnb2 and mCherry from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 for sgRNA and hepatocyte-specific promoter (HS…

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge