We narrowed to 12,237 results for: nsf

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pAAV-CMV-BD10-SAS620-3’dCas9-VPR-synpA

Plasmid

#216323

Purpose

Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Cas9-VPR + splice acceptor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD

Plasmid

#204359

Purpose

AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDq-mCherry-P2A-HA-KORD

Use

AAV

Tags

HA (for KORD) and mCherry (for miniDq)

Mutation

For miniDq, the third intracellular loop (ICL3) o…

Promoter

hSyn

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-ABE C-terminal

Plasmid

#137178

Purpose

AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Promoter

CMV

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-hSynI-rtTAV16

Plasmid

#102367

Purpose

AAV vector to drive TET-ON activator expression from human synapsin I promoter

Depositor

Tetsuo Yamamori

Article

Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

Insert

rtTAV16

Use

AAV

Expression

Mammalian

Mutation

rtTA variant reported in Gene Therapy (2006) 13, …

Promoter

human synapsin I promoter

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-UbC-eGFP

Plasmid

#62518

Purpose

Expresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.

Depositor

Jae Lee, Pantelis Tsoulfas

Article

Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

hUbC

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFP

Plasmid

#178707

Purpose

AAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

Dreadd-hM3Dq-HA-P2A-tBFP

Use

AAV

Promoter

hSyn

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

Plasmid

#101060

Purpose

AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC588 - pAAV c-fos Nuc-iRFP

Plasmid

#59132

Purpose

An AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Nuclear-localized Infrared Fluorescent Protein

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

mFos

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDRM18 LTN (Luciferase tdTomato Neo)

Plasmid

#174721

Purpose

Expresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

Firefly Luciferase
tdTomato
aph

Use

Lentiviral and Luciferase

Tags

E2A linker and P2A linker

Expression

Mammalian

Promoter

MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP13A3 WT

Plasmid

#195849

Purpose

Expresses wild-type Homo sapiens ATP13A3

Depositor

Veerle Baekelandt

Article

Houdou et al Biomolecules. 2023 Feb 9;13(2):337. doi:

Insert

ATPase 13A3 (ATP13A3 Human)

Use

Lentiviral

Mutation

D498N, L956P, M850I, V855M, R858H, L675V

Promoter

CMV

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

Plasmid

#207860

Purpose

AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-mCherry

Plasmid

#137134

Purpose

Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDRM209 LTP (Luciferase tdTomato Puro)

Plasmid

#174723

Purpose

Expresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

Firefly Luciferase
tdTomato
pac

Use

Lentiviral and Luciferase

Tags

E2A linker and T2A linker

Expression

Mammalian

Promoter

MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-p300

Plasmid

#191764

Purpose

Expression of EGFP fused to core p300 histone acetyltransferase

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

FRB-EGFP-p300 core (EP300 Human)

Tags

EGFP (N-terminal of p300 core) and FRB (N-termina…

Expression

Bacterial and Mammalian

Promoter

EF1alpha

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChrimsonR-tdT

Plasmid

#59171

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA

Plasmid

#216320

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice donor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA

Plasmid

#216321

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice acceptor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-rsChRmine-oScarlet-WPRE

Plasmid

#183522

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-oScarlet

Use

AAV

Mutation

I146M/G174S

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAJE3-E7

Plasmid

#214359

Purpose

Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

Depositor

Ryan Mehl

Article

Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

Inserts

2nd generation Tet2 "E7" aminoacyl tRNA synthetase
Lpp promoted M. jannaschii tRNA
Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
Orthogonal "D4II" ColE1 origin

Expression

Bacterial

Promoter

AmpR and lpp

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

Plasmid

#207878

Purpose

AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

Depositor

Yeh-Hsing Lao

Article

Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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