We narrowed to 90,070 results for: Mal

1. pMXs-WT-ATPIF1

   Plasmid

   #85403

   Purpose

   Retroviral vector expressing wild type human ATPIF1

   Depositor

   David Sabatini

   Article

   Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

   Insert

   ATPIF1 (ATP5IF1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Wildtype

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pCMV-dCas12a-A3A-N57G

   Plasmid

   #193639

   Purpose

   Expression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3A-N57G, EGFP
   

   Expression

   Mammalian

   Mutation

   hAPOBEC3A-N57G

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-dCas12a-A3A-Y130F

   Plasmid

   #193640

   Purpose

   Expression of base editor dCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3A-Y130F, EGFP
   

   Expression

   Mammalian

   Mutation

   hAPOBEC3A-Y130F

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV/myc/mito-mutant-hogg1

   Plasmid

   #18708

   Depositor

   David Sidransky

   Article

   Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

   Insert

   8-oxoguanine DNA glycosylase (OGG1 Human)
   

   Expression

   Mammalian

   Mutation

   Amino acid 229, Arginine to Glutamine

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

5. FUW-TetO-Lin28

   Plasmid

   #60345

   Purpose

   Mammalian Expression, Lentiviral

   Depositor

   Rudolf Jaenisch

   Article

   Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

   Insert

   Lin28 (Lin28a Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Minimal CMV + TetO

   Available Since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

6. U6_sgRNA_CAG_hSt1Cas9_LMD9

   Plasmid

   #110626

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and hU6

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOS010: pLX304-CheRiff-GFP

   Plasmid

   #62986

   Purpose

   Lentivirus production of CheRiff channelrhodopsin actuator

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   CheRiff-eGFP
   

   Use

   Lentiviral; Gateway destination vector

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pODN-H2BC11-mR2

   Plasmid

   #183866

   Purpose

   Repair template for the C-terminal tagging of H2B histones with mRuby2 in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 homology arms with linker-mRuby2 (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mRuby2

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pODN-mR2-MYH10

   Plasmid

   #183869

   Purpose

   Repair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   MYH10 homology arms with mRuby2-linker (MYH10 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA

    Plasmid

    #154775

    Purpose

    plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAA in GFP reporter, UUA anticodon and G10A, …

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

    Plasmid

    #163572

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9-UdgX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AID-NG-CBE

    Plasmid

    #227416

    Purpose

    C-to-T base editing in mammalian cells

    Depositor

    Yang Sun

    Article

    Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

    Insert

    AID-nSpCas9-NG-2xUGI (AICDA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AID-N51A-NG-CBE

    Plasmid

    #227417

    Purpose

    C-to-T base editing in mammalian cells

    Depositor

    Yang Sun

    Article

    Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

    Insert

    AID-N51A-nSpCas9-NG-2xUGI (AICDA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459-HypaCas9-ACTB_sgRNA

    Plasmid

    #183885

    Purpose

    pX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ACTB sgRNA spacer (ACTB Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCGN K-Ras 12V, 188L

    Plasmid

    #14722

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

    Insert

    K-ras (KRAS Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    12V, 188L

    Available Since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1_hE2F7mg

    Plasmid

    #73073

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM60-FLAG-SLC38A9.1 (1-119aa)

    Plasmid

    #71862

    Purpose

    lentiviral stable expression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized, first 119 amino acids

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pTBL680 CHYRON3 integration construct

    Plasmid

    #126448

    Purpose

    To integrate the CHYRON3 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON3 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQLL-Tc1-8B_DR[EF1α-copGFP]

    Plasmid

    #218327

    Purpose

    Donor plasmid of Tc1-8B_DR Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

    Plasmid

    #71217

    Purpose

    dCas9 fused to human DNMT3B catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3B catalytic domain
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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