We narrowed to 15,981 results for: LEA

1. NLS-LgBiT-GAI

   Plasmid

   #183405

   Purpose

   Expresses nuclear-localized LgBiT-GAI

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   LgBiT-GAI
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMVTO-gfp-Kir2.1-HA-tevs-KKYL

   Plasmid

   #179706

   Purpose

   TEVP-inducible GFP-conjugated Kir2.1 RELEASE with KKXX motif

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   Kir2.1
   

   Expression

   Mammalian

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRMCD111

   Plasmid

   #172957

   Purpose

   pRMCD77 encoding Wzz mutant G311A

   Depositor

   Renato Morona

   Article

   Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

   Insert

   Shigella flexneri wzz G311A mutant gene
   

   Expression

   Bacterial

   Mutation

   D168G and N33D- please see depositor comment

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRMCD113

   Plasmid

   #172959

   Purpose

   pRMCD77 encoding Wzz mutant G305A/G311A

   Depositor

   Renato Morona

   Article

   Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

   Insert

   Shigella flexneri wzz G305A/G311A mutant gene
   

   Expression

   Bacterial

   Mutation

   G305A/G311A, A208V - please see depositor comments

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSETA_crG18

   Plasmid

   #172930

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 18x GSG

   Depositor

   Arnold Boersma

   Article

   Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

   Insert

   CrowdingSensorG18
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEPQD0CM0549

   Plasmid

   #162300

   Purpose

   Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   N-terminal tag, SUMO-[TEV cleavage site]
   

   Use

   Synthetic Biology

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEPQD0CM0550

   Plasmid

   #162301

   Purpose

   Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   N-terminal tag, HiBiT-SUMO-[TEV cleavage site]
   

   Use

   Synthetic Biology

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPMY0SP0002

   Plasmid

   #162304

   Purpose

   Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   N-terminal tag, 6xHis tag-[HRV 3C cleavage site]
   

   Use

   Synthetic Biology

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEPQD0CM0551

   Plasmid

   #162303

   Purpose

   Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   N-terminal tag, HiBiT-[S-tag]-[TEV cleavage site]
   

   Use

   Synthetic Biology

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEPQD0CM0547

    Plasmid

    #162298

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, TrxA-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPQD0CM0546

    Plasmid

    #162297

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, MBP-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEPQD0CM0544

    Plasmid

    #162295

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, MBP-[Factor Xa cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEPQD0CM0543

    Plasmid

    #162294

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, GST-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPQD0CM0541

    Plasmid

    #162292

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, GST-[thrombin cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-nls

    Plasmid

    #108882

    Purpose

    Multisite gateway vector for 5' tagging with an N-terminal nuclear localisation sequence

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    nls
    

    Use

    Multisite gateway

    Available Since

    Oct. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMW-PahZ1 KT-1

    Plasmid

    #141134

    Purpose

    Expresses in BL-21 (DE3) cells to produce the poly(aspartic acid) hydrolase-1 from Sphingomonas Sp. KT-1 which selectively cleaves the beta linkages in thermally synthesized poly(aspartic acid).

    Depositor

    Mitch Weiland

    Article

    Bolay et al Journal of Chemical Education

    Insert

    PahZ1
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Not the full-length gene. PahZ1 fragment starts a…

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGBW-m4258304

    Plasmid

    #154803

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;TRX

    Expression

    Mammalian

    Mutation

    H. sapiens recode 1

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pGBW-m4258292

    Plasmid

    #154788

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;S-tag

    Expression

    Mammalian

    Mutation

    H. sapiens recode 1

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGBW-m4133484

    Plasmid

    #152973

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHis-003

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pGBW-m4133491

    Plasmid

    #152964

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHis-003

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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