We narrowed to 11,592 results for: Vars;

1. pAcBac1-BacMam-4x-PyOtR

   Plasmid

   #217364

   Purpose

   Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

   Insert

   4 copies of pyrrolysyl-tRNA mutant PyOtR
   

   Use

   Baculoviral

   Expression

   Insect and Mammalian

   Promoter

   U6

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-CMV-GCaMP6-210

   Plasmid

   #91778

   Purpose

   Mammalian expression of low affinity calcium-sensing fluorescent protein

   Depositor

   GENIE Project, Douglas Kim

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   GCaMP6-210
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10

   Plasmid

   #162384

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8m
   

   Tags

   6xHis

   Expression

   Insect

   Mutation

   A25G F286Y

   Promoter

   hsp70

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8f-p10

   Plasmid

   #162385

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8f
   

   Tags

   6xHis

   Expression

   Insect

   Mutation

   Q315L

   Promoter

   hsp70

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH6-TEV-spTorA-GFP(C)

   Plasmid

   #169041

   Purpose

   GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

   Depositor

   Siegfried Musser

   Article

   Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

   Insert

   H6-spTorA-GFP
   

   Tags

   6xHis, TEV cleavage site, and spTorA (signal pept…

   Expression

   Bacterial

   Mutation

   The mut3 GFP variant with 5 additional mutations …

   Promoter

   araBAD

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-DIO-trkB.DN-mCherry

   Plasmid

   #121502

   Purpose

   Expresses trkB.T1 fused with mCherry in a cre dependent manner.

   Depositor

   Marie Carlén

   Article

   Guyon et al J Neurosci. 2021 Feb 15. pii: JNEUROSCI.

   Insert

   trkB.T1 (Ntrk2 Rat)
   

   Use

   AAV and Cre/Lox

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEMS-Tom20-mEGFP-reHaloTagS

   Plasmid

   #200189

   Purpose

   Tom20 with reversible HaloTagS variant for fluorescent labeling

   Depositor

   Jacob Piehler

   Article

   Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

   Insert

   Tom20 (TOMM20 Human)
   

   Tags

   mEGFP and reHaloTagS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCX0393

   Plasmid

   #205984

   Purpose

   Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Inserts

   Ga0116245_10002768 candidate type VII proteins
   Cas14
   Cas5
   truncated CRISPR array
   

   Tags

   14xHis and TwinStrep-tag

   Expression

   Bacterial

   Promoter

   T7 (proteins), pJ23119 (crRNA)

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-ITR-EcYtR(NGS-6)-mCherry

   Plasmid

   #217363

   Purpose

   Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   E. coli tyrosine tRNA mutant, "NGS-6"
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAX APPs-751-alpha

    Plasmid

    #30149

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein - soluble (APP Human)
    

    Expression

    Mammalian

    Mutation

    splice variant 751; amino acids 1–612

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1-mPCSK3 H194L

    Plasmid

    #122689

    Purpose

    Expresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    

    Expression

    Mammalian

    Mutation

    Changed Histidine 194 to Leucine

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-mPCSK3 S368A

    Plasmid

    #122680

    Purpose

    Expresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    

    Expression

    Mammalian

    Mutation

    changed Serine 368 to Alanine

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2R3a-3xVenusYFP-OcsT

    Plasmid

    #71271

    Purpose

    Entry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    3 times VenusYFP
    

    Use

    Gateway

    Tags

    octaline synthase terminator

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. RIP7-RLuc-YFP-pBS

    Plasmid

    #72482

    Purpose

    Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals

    Depositor

    Vincent Poitout

    Article

    Ghislain et al Endocrinology. 2015 Dec 15:en20151550.

    Inserts

    rat ins2 gene promoter (Ins2 Rat)
    luciferase
    eYFP
    

    Use

    Luciferase

    Tags

    eYFP and renilla luciferase

    Expression

    Mammalian

    Mutation

    eYFP varient

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET19-MTdTc302

    Plasmid

    #207458

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTc302 (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid

    #141236

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    NES-jRGECO1a
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    6xHIS tag and nuclear export signal

    Promoter

    human elongation factor-1 alpha (EF-1 alpha)

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-hRedOpsin-fCD200

    Plasmid

    #164425

    Purpose

    AAV plasmid expressing full-length CD200 in photoreceptors

    Depositor

    Connie Cepko

    Article

    Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

    Insert

    CD200 (Cd200 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human red opsin

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS423-mitoT

    Plasmid

    #174525

    Purpose

    Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

    Depositor

    Oleh Khalimonchuk

    Article

    Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

    Insert

    Yeast mitochondrial intermembrane tether
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Yeast

    Promoter

    MET25

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTH728-CEN-RLuc/maxCFLuc

    Plasmid

    #38212

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3019_pETcon-SARS-CoV-2-RBD_E484K

    Plasmid

    #184404

    Purpose

    yeast surface display of the SARS-CoV-2 Eta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    E484K

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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