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A novel interaction between RAD23A/B and Y-family DNA polymerases.
Ashton NW, Jaiswal N, Cestari Moreno N, Semenova IV, D'Orlando DA, Teatin Latancia M, McIntyre J, Woodgate R, Bezsonova I
J Mol Biol. 2023 Nov 5:168353. doi: 10.1016/j.jmb.2023.168353. PubMed Article

Plasmids from Article

ID Plasmid Purpose
201438pCMV6-AN-DDK_WT RAD23AExpresses human RAD23A with an N-terminal FLAG tag in mammalian cells
201439pCMV6-AN-DDK_WT RAD23BExpresses human RAD23B with an N-terminal FLAG tag in mammalian cells
201440pCMV6-AN-HA_WT POLIExpresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells
201444pJM919_WT His POLILow copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tag
201445pJRM919_WT aa 1-419 His POLILow copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLI
201456pGEX-6P-1_WT RAD23AExpresses human RAD23A with an N-terminal GST tag in E. coli
201457pGEX-6P-1_WT RAD23BExpresses human RAD23B with an N-terminal GST tag in E. coli
201458pCMV6-AN-HA_WT RAD23AExpresses human RAD23A with an N-terminal HA tag in mammalian cells
201459pCMV6-AN-HA_WT RAD23BExpresses human RAD23B with an N-terminal HA tag in mammalian cells
201460pCMV6-AN-DDK_WT RAD23A_aa 111-363 (delta UBL domain)Expresses a mutant form of human RAD23A that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.
201463pCMV6-AN-DDK_WT RAD23A_delta aa 161-201 (delta UBA1 domain)Expresses a mutant form of human RAD23A that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.
201464pCMV6-AN-DDK_WT RAD23A_delta aa 230-285 (delta XPC-binding domain)Expresses a mutant form of human RAD23A that lacks the XPC-binding domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.
201465pCMV6-AN-DDK_WT aa 1-317 RAD23A (delta UBA2 domain)Expresses a mutant form of human RAD23A that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.
201466pCMV6-AN-DDK_WT RAD23A_delta aa 111-136Expresses a mutant form of human RAD23A lacking residues 111-136. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.
201467pCMV6-AN-DDK_WT RAD23A_delta aa 137-160Expresses a mutant form of human RAD23A lacking residues 137-160. Variant of plasmid pCMV6-AN-DDK_WT RAD23A
201468pCMV6-AN-DDK_WT RAD23A_delta aa 202-230Expresses a mutant form of human RAD23A lacking residues 202-230. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.
201469pCMV6-AN-DDK_WT RAD23A_delta aa 287-316Expresses a mutant form of human RAD23A lacking residues 287-316. Variant of plasmid pCMV6-AN-DDK_WT RAD23A
201545pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)Expresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
201546pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)Expresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
201547pCMV6-AN-DDK_WT RAD23B_delta aa 276-339 (delta XPC-binding domain)Expresses a mutant form of human RAD23B that lacks the XPC-binding domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
201548pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)Expresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
201549pet28b_His_RAD23A-UBA1Expresses UBA1 of human RAD23A in E. coli
201550pet28b-His_RAD23A-UBA2Expresses UBA2 of human RAD23A in E. coli
201574pCMV6-AN-DDK_S172A_M173A RAD23AExpresses a variant of human RAD23A containing mutations S172A and M173A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A
201575pCMV6-AN-DDK_V195A RAD23AExpresses a variant of human RAD23A containing mutation V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A
201576pCMV6-AN-DDK_S172A_M173A_V195A RAD23AExpresses a variant of human RAD23A containing mutations S172A, M173A and V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A
201577pCMV6-AN-DDK_C344A RAD23AExpresses a variant of human RAD23A containing mutation C344A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A
201578pCMV6-AN-DDK_F354A RAD23AExpresses a variant of human RAD23A containing mutation F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A
201579pCMV6-AN-DDK_C344A_F354A RAD23AExpresses a variant of human RAD23A containing mutations C344A and F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A
201580pCMV6-AN-DDK_V222A RAD23BExpresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
201581pCMV6-AN-DDK_F400A RAD23BExpresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
201582pCMV6-AN-HA_aa 1-420 POLIExpresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells
201583pCMV6-AN-HA_aa 1-449 POLHExpresses the catalytic domain (aa 1-449) of human DNA polymerase eta with a HA tag in mammalian cells
201584pCMV6-AN-HA_WT POLKExpresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cells
201585pCMV6-AN-HA_aa 1-542 POLKExpresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells
201586pCMV6-AN-HA_WT REV1Expresses human DNA repair protein REV1 with an N-terminal HA tag in mammalian cells
201587pCMV6-AN-HA_aa 321-843 REV1Expresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cells

Antibodies from Article