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We narrowed to 18,123 results for: multi

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Showing: 10661 - 10680 of 18123 results

  1. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid
    #200910
    Purpose
    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    3XHA_den1FnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Mutation
    Changed Glutamic Acid to Arginine at 1369 position
    Promoter
    CBH
    Available Since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200910-plasmid-map-sequence-id-407164

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  2. pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE

    Plasmid
    #202198
    Purpose
    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotor
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Insert
    stChrimsonR, PdCO
    Use
    AAV
    Tags
    EGFP and Rho1D4
    Expression
    Mammalian
    Promoter
    CaMKIIα minimal promotor (0.4kb)
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202198-plasmid-map-sequence-id-397077

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  3. LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode

    Plasmid
    #170228
    Purpose
    Barcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    Map2k7-MAPK9 fusion (MAPK9, Map2k7 Human, Mouse)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    Fusion
    Promoter
    EF1a
    Available Since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170228-plasmid-map-sequence-id-334409

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  4. NbPT5b-p3

    Plasmid
    #160001
    Purpose
    Multigene construct containing the betalain pigment biosynthetic genes under the control of the NbPT5b promoter for visualisation of arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Inserts
    Bar (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    Expression
    Plant
    Promoter
    Agrobacterium tumefaciens Nopaline synthase Promo…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160001-plasmid-map-sequence-id-323057

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  6. pFUSEss-CHIg-mG1_CH1h-3

    Plasmid
    #105850
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH1 and hinge.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG1 heavy chain, IgG3 heavy chain (Ighg1, Ighg3 Mouse)
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG1 with CH1 and hin…
    Promoter
    hEF1-HTLV prom
    Available Since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    105850-plasmid-map-sequence-id-206012

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  10. pLK06Hpp-Fab CR3009

    Plasmid
    #239861
    Purpose
    Plasmid for simultaneous Fab and GFP expression from separate Lac promoters for online monitoring of induction with a GFP sensor station. Fab is expressed as a fusion with alkaline phosphatase.
    Depositor
    Urpo Lamminmäki
    Article
    Korkiakoski et al Protein Expr Purif. 2025 Aug 28:106808.
    Inserts
    Fab (antigen binding fragment) anti-nucleocapsid protein of SARS-COV viruses 1 and 2 (N, N Human)
    GFP with F64L, S65T, Q80R, M153T and V163A mutations
    Tags
    Hexahistidine tag and bacterial alkaline phosphat…
    Expression
    Bacterial
    Mutation
    F64L, S65T, Q80R, M153T and V163A
    Promoter
    Lac promoter
    Available Since
    Sept. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239861-plasmid-map-sequence-id-485412

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  11. pAED2

    Plasmid
    #234599
    Purpose
    Fluorescent reporter of the SOS response
    Depositor
    Tanel Tenson
    Article
    Kaldalu et al NPJ Antimicrob Resist. 2025 Apr 11;3(1):
    Inserts
    mScarlet-I
    gfp-mut2
    Use
    Reporter
    Expression
    Bacterial
    Mutation
    GFPmut2 was derived from avGFP with the following…
    Promoter
    Ptet+dnaK P1 and cda
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234599-plasmid-map-sequence-id-467741

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  12. pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-Flag

    Plasmid
    #210820
    Purpose
    Mammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)
    Depositor
    Luke Chao
    Article
    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7
    Inserts
    Prominin 1 (PROM1 Human)
    Protocadherin 21 (CDHR1 Human)
    Tags
    Myc, 3xFlag and TwinStrep, 10xHis
    Expression
    Mammalian
    Promoter
    CMV and CMV / IRES2
    Available Since
    July 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210820-plasmid-map-sequence-id-417732

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  13. pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)

    Plasmid
    #185447
    Purpose
    Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cells
    Depositor
    Ahmet Yildiz
    Article
    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.
    Inserts
    TRF1 (TERF1 Human)
    POT1 (POT1 Human)
    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    Tags
    MBP, YBBR, and ZZ
    Expression
    Insect
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    185447-plasmid-map-sequence-id-364982

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  15. AAV-KrasG12A/sgKras/Cre

    Plasmid
    #99849
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99849-plasmid-map-sequence-id-193789

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  16. AAV-KrasG13C/sgKras/Cre

    Plasmid
    #99856
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99856-plasmid-map-sequence-id-193794

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  19. pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

    Plasmid
    #187959
    Purpose
    Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Inserts
    Blasticidin resistance
    FKBP12_F36V
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187959-plasmid-map-sequence-id-373708

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  20. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

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Showing: 10661 - 10680 of 18123 results