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ptdEos-CLC
(Plasmid #38009)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38009 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEYFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6028
  • Modifications to backbone
    EYFP coding sequence removed with NheI (5') and EcoRI (3').
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Clathrin, light polypeptide (LCa)
  • Alt name
    Clta
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    657
  • GenBank ID
    NM_001080385
  • Entrez Gene
    Clta (a.k.a. AV026556, Lca)
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer CMV primer
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Eos FP, tandem dimer
  • Alt name
    tdEos
  • Insert Size (bp)
    1348
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    tdEos was derived from tdEos-Vinculin, a kind gift of Dr. Michael W. Davidson (Florida State University). Clathrin light chain was a kind of gift of Dr. James H. Keen (Kimmel Cancer Center).
  • Terms and Licenses

Depositor Comments

Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function. It is also missing its stop codon, so the peptide "PGPGSTGSR*" from the backbone is appended to the C-terminus of the CLC. The affect of this peptide on protein function is unknown.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ptdEos-CLC was a gift from Xiaowei Zhuang (Addgene plasmid # 38009 ; http://n2t.net/addgene:38009 ; RRID:Addgene_38009)
  • For your References section:

    Fast, three-dimensional super-resolution imaging of live cells. Jones SA, Shim SH, He J, Zhuang X. Nat Methods. 2011 Jun;8(6):499-508. Epub 2011 May 8. 10.1038/nmeth.1605 PubMed 21552254