Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 16,278 results for: nes

Clear keywords
Showing: 10701 - 10720 of 16278 results

  4. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid
    #196079
    Purpose
    Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA targeting Adam10
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Cloned using SapI sites - destroyed after inserti…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196079-plasmid-map-sequence-id-388996

    Enlarge

  5. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid
    #196085
    Purpose
    Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA targeting Adam10
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Cloned using SapI sites - destroyed after inserti…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196085-plasmid-map-sequence-id-388624

    Enlarge

  6. pcDNA3-mCherry-TSC2-NLS

    Plasmid
    #192441
    Purpose
    Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-TSC2-NLS (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192441-plasmid-map-sequence-id-379077

    Enlarge

  8. pJET-07-mNeonGreen-R11a

    Plasmid
    #179890
    Purpose
    Donor with homologous arms for Rab11a to knock in mNeonGreen
    Depositor
    Patrick Caswell
    Publication
    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB11A, member RAS oncogene family (RAB11A Human)
    Use
    CRISPR; Donor for homologous based knock in
    Tags
    mNeonGreen
    Expression
    Bacterial and Mammalian
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179890-plasmid-map-sequence-id-357211

    Enlarge

  13. PB-PE VQR

    Plasmid
    #162797
    Purpose
    All-in-one prime editor piggyBac transposon, VQR variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    CAG
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    162797-plasmid-map-sequence-id-320036

    Enlarge

  14. pRC/CMV-TYK21054-5FF-VSV

    Plasmid
    #139347
    Purpose
    Expression of TYK2 protein mutated in the two tyrosines (Y1054F/Y1055F) of the activation loop
    Depositor
    Sandra Pellegrini
    Publication
    Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    Expression
    Mammalian
    Mutation
    V362F-Y1054F-Y1055F
    Promoter
    CMV
    Available since
    June 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    139347-plasmid-map-sequence-id-291878

    Enlarge

  15. pCI.neo-rPAM1-M314I

    Plasmid
    #145802
    Purpose
    expresses catalytically inactive rat PAM-1 in mammalian cells
    Depositor
    Betty Eipper, Richard Mains
    Publication
    Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
    Expression
    Mammalian
    Mutation
    changed methionine 314 to isoleucine to inactivat…
    Available since
    June 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    145802-plasmid-map-sequence-id-299344

    Enlarge

  16. NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

    Plasmid
    #89994
    Purpose
    Donor template for generation of NGN3-ntdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)
    Use
    CRISPR
    Promoter
    none
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    89994-plasmid-map-sequence-id-180699

    Enlarge

  17. pRC/CMV-TYK2K930R-FF-VSV

    Plasmid
    #139348
    Purpose
    Expression of kinase-dead TYK2-K930R missing the two tyrosines at the activation loop
    Depositor
    Sandra Pellegrini
    Publication
    Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    Tags
    VSV
    Expression
    Mammalian
    Mutation
    V362F-K930R-Y1054F-Y1055F
    Promoter
    CMV
    Available since
    March 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    139348-plasmid-map-sequence-id-275739

    Enlarge

  19. CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

    Plasmid
    #111923
    Purpose
    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ
    Depositor
    Takeharu Nagai
    Publication
    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CeNL(Ca2+)_19μ
    Tags
    CoxVIIIx2
    Expression
    Mammalian
    Mutation
    E67D, F92W, E104D, D133E at CaM
    Promoter
    CMV
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111923-plasmid-map-sequence-id-222573

    Enlarge

  20. mCherry-FKBP-SAC1C389S-HLx8

    Plasmid
    #108138
    Purpose
    Recruitable inactive SAC1 with extended linker
    Depositor
    Gerry Hammond
    Publication
    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
    Expression
    Mammalian
    Mutation
    SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)
    Promoter
    CMV
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108138-plasmid-map-sequence-id-207729

    Enlarge

Showing: 10701 - 10720 of 16278 results