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pEYRC-C1A

Plasmid

#127752

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

RFP, CFP, Convergent Orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAc-Cdc3-Cdc10-SNAP

Plasmid

#131161

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Article

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

Cdc3 + Cdc10-SNAP

Use

Bicistronic

Tags

Cdc10: SNAP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace1_Q89D_D100N_E206V-HaloTag

Plasmid

#129255

Purpose

Express Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Mac_Q139D_D150N_E258V-HaloTag-ST

Plasmid

#129264

Purpose

Express soma-localized Mac_Q139D_D150N_E258V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Mac_Q139D_D150N_E258V-HaloTag-ST

Expression

Mammalian

Mutation

Q139D,D150N,E258V

Promoter

CAG

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag-ST

Plasmid

#129263

Purpose

Express soma-localized QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

QuaAr3_Q95D_H106N_E214V-HaloTag-ST

Expression

Mammalian

Mutation

Q95D, H106N, E214V

Promoter

CAG

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace1_Q89D_D100N_E206V-HaloTag-ST

Plasmid

#129260

Purpose

Express soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag-ST

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Mac_Q139D_D150N_E258V-HaloTag

Plasmid

#129259

Purpose

Express Mac_Q139D_D150N_E258V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Mac_Q139D_D150N_E258V-HaloTag

Expression

Mammalian

Mutation

Q139D,D150N,E258V

Promoter

CAG

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag

Plasmid

#129258

Purpose

Express QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

QuaAr3_Q95D_H106N_E214V-HaloTag

Expression

Mammalian

Mutation

Q95D, H106N, E214V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH7-8

Plasmid

#124991

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH7-8 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH7-8 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pB-puro-mU6-RtcAshRNA1

Plasmid

#126613

Purpose

Expresses shRNA against human RtcA under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

RtcA shRNA-1

Use

RNAi

Promoter

mouse U6 promoter

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH7-11

Plasmid

#124993

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH7-11 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH7-11 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH7-9

Plasmid

#124992

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH7-9 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH7-9 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH7-6

Plasmid

#124990

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH7-6 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH7-6 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-3

Plasmid

#124983

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-3 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

TCR-HH5-3

Use

retroviral

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pB-puro-mU6-RtcAshRNA2

Plasmid

#126614

Purpose

Expresses shRNA against human RtcA under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

RtcA shRNA-2

Use

RNAi

Promoter

Mouse U6 promoter

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-GeNL-Ca

Plasmid

#101003

Purpose

Construct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis expression

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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Smp_031880-Cd4-bio-His

Plasmid

#120602

Purpose

Expression of recombinant S. mansoni protein

Depositor

Gavin Wright

Article

Cécile et al bioRXiv

Insert

Smp_031880 (Smp_031880 )

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF704

Plasmid

#121658

Purpose

U6-sgRNA EFS-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF712

Plasmid

#121663

Purpose

U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG030

Plasmid

#113993

Purpose

Level 0 module. IPTG-inducible T7RNAP promoter for BL21 protein purification

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

IPTG-inducible T7RNAP promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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