We narrowed to 64,742 results for: cell;

1. myc-BioID-Rab7

   Plasmid

   #121049

   Purpose

   Expresses myc tagged BioID and human Rab7a in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

   Inserts

   BioID
   Rab7A
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R118G (highly promiscuous form)

   Promoter

   CMV

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPLtetO-FtsZ

   Plasmid

   #89656

   Purpose

   Plasmid for titrating synthesis of FtsZ with doxycycline

   Depositor

   Uwe Sauer

   Article

   Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

   Insert

   GTP-binding tubulin-like cell division protein
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLib6.4B-Consensus

   Plasmid

   #176678

   Purpose

   Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFX-EGFP-Rab11

   Plasmid

   #174460

   Purpose

   Express iRFP with recycling endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with recycling endosome-targeting sequence
   

   Tags

   small GTPase (Rab11)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. eGFP-KIF5A

   Plasmid

   #172201

   Purpose

   Expresses KIF5A tagged with eGFP in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

   Insert

   KIF5A (Kif5a Mouse)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-mCherry-CyrCR1.miR16

   Plasmid

   #128747

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xFLAG-dCas9/MSCV-EGFP

   Plasmid

   #82613

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g

   Insert

   3xFLAG-dCas9
   

   Use

   Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFX-H2B-Venus

   Plasmid

   #175840

   Purpose

   Express Venus with nucleus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with nucleus-targeting sequence
   

   Tags

   H2B (human histone 2B)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-UBC-TagRFP-T-ps

   Plasmid

   #178656

   Purpose

   Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

   Insert

   TagRFP-T with palmitoylation signal at N-terminus
   

   Use

   Lentiviral

   Tags

   Palmitoylation signal

   Promoter

   Ubc

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-C1-BBS5

    Plasmid

    #218714

    Purpose

    Expresses N-terminally EGFP-tagged BBS5 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS5 (BBS5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)

    Plasmid

    #191145

    Purpose

    Expression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    3UTR(Bdnf)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP

    Plasmid

    #153008

    Purpose

    Expresses glucagon peptide on mammalian cell surface

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    Glucagon-ICAM1-pre-mGRASP
    

    Tags

    3x HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-CMV 3xFLAG-LC3C-GFP

    Plasmid

    #123110

    Purpose

    Expresses 3xFLAG-LC3C-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3C

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
    

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFX-H2B-iRFP

    Plasmid

    #175842

    Purpose

    Express iRFP with nucleus-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    iRFP with nucleus-targeting sequence
    

    Tags

    H2B (human histone 2B)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5RS-HERCD533

    Plasmid

    #65224

    Purpose

    Expression of dominant negative EGFR in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Redemann et al Mol Cell Biol. 1992 Feb;12(2):491-8.

    Insert

    HERCD533 (EGFR Human)
    

    Expression

    Mammalian

    Mutation

    deleted AA 654-1186

    Promoter

    CMV

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

16. EFF-1::V5

    Plasmid

    #132961

    Purpose

    Expresses EFF-1 A full length protein in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

    Insert

    EFF-1::V5
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF-pIgH-ESCargo*

    Plasmid

    #140159

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-DsRed-Express2-FKBP(LV)(C22V)
    

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. NSK024 SFFV-mCherry-SG(p53R248Q.s2)SG-EGFP (FLP-IN)

    Plasmid

    #191169

    Purpose

    Expression of a sensor for p53 mutant R248Q in mammalian cells; mCherry marker, EGFP output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:p53(R248Q).s2:EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJC2-UMPS-3xFLAG

    Plasmid

    #87975

    Purpose

    3rd generation lentiviral vector for Expression of UMPS in mammalian cells

    Depositor

    David Sabatini

    Article

    Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

    Insert

    UMPS (UMPS Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tet-O-FUW-Nkx6.1

    Plasmid

    #45846

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

    Insert

    Nkx6.1 (Nkx6-1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    tet-ON

    Available Since

    July 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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