We narrowed to 2,702 results for: puc19

1. AbVec2.0-mIghg3

   Plasmid

   #127160

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotype

   Depositor

   Hedda Wardemann

   Insert

   Ighg3 (Ighg3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC119

   Plasmid

   #50010

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Vieira et al Methods Enzymol. 1987;153:3-11.

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)

   Plasmid

   #191391

   Purpose

   Donor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hCdt1(30-120)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPATZ1-donor

   Plasmid

   #132522

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   PATZ1 (PATZ1 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   none

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. hSLBP_mNG_FS_Donor

   Plasmid

   #191387

   Purpose

   Donor for C-terminal tagging of endogenous hSLBP

   Depositor

   Yannick Doyon

   Insert

   mNeonGreen(mNG) + FS(3xFLAG_TwinStrep)Tag for hSLBP tagging at the C-terminus (SLBP Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUA97

   Plasmid

   #69340

   Purpose

   It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy

   Depositor

   Oliver Hobert

   Insert

   ceBFP SEC 3xFlag
   

   Use

   Tags

   3xFlag and ceBFP (mTagBFP)

   Expression

   Worm

   Mutation

   sqt-1(e1350)

   Promoter

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pINER5

   Plasmid

   #185895

   Purpose

   In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 25, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pBV58

   Plasmid

   #204603

   Purpose

   ERG11-3X Minidegron-5X FLAG-HIS3MX6

   Depositor

   Scott Moye-Rowley

   Insert

   Candida glabrata ERG11 (ERG11 )
   

   Use

   Tags

   auxin-inducible degron

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRARB-donor

   Plasmid

   #132517

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   RARB (RARB Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   none

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAW8-aid-3HA-Turg1:kanMX6

    Plasmid

    #169355

    Purpose

    C-terminal aid-3HA degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    3XHA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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