We narrowed to 11,215 results for: Kars;

1. pBabe NEO mRFP1

   Plasmid

   #98397

   Purpose

   Retroviral vector for constitutive mRFP1 expression

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   mRFP1
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pWJ66

   Plasmid

   #46570

   Purpose

   Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   dcas9-w
   CRISPR array
   

   Use

   CRISPR

   Tags

   rpoZ

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pN1-CMV-Aff6_V18FdN-IntC-mCherryC-T2A-EGFP (C2)

   Plasmid

   #210725

   Purpose

   NIR optogenetic module for conditional protein splicing of split mCherry (C-part)

   Depositor

   Vladislav Verkhusha

   Article

   Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

   Insert

   C2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCherry-FRB

   Plasmid

   #25920

   Depositor

   Klaus Hahn

   Article

   Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

   Insert

   FRB (MTOR Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. flag-RapR-PTP1B

   Plasmid

   #188656

   Purpose

   Rapamycin regulated PTP1B with flag tag

   Depositor

   Andrei Karginov

   Article

   Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   PTP1B
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pG4

   Plasmid

   #162605

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pG2

   Plasmid

   #162603

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-WIPI3

   Plasmid

   #42527

   Depositor

   James Hurley

   Article

   Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

   Insert

   WIPI3 (WDR45B Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Mammalian

   Mutation

   gene sequence is codon optimized for expression i…

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pACAGW-ChR2-Venus-AAV

   Plasmid

   #20071

   Purpose

   AAV-mediated expression of ChR2-Venus for neuronal excitation

   Depositor

   Karel Svoboda

   Article

   Leopoldo et al Nature. 2009 Jan 18.

   Has Service

   AAV1 and AAV9

   Insert

   Channelrhodopsin-2 (CHLRE_02g085257v5 Chlamydomonas reinhardtii)
   

   Use

   AAV

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCas9

    Plasmid

    #162606

    Purpose

    Pre-express Cas9 in yeast

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHACK(Gal4)-DONR(T2A-LexAGAD)

    Plasmid

    #194769

    Purpose

    To insert T2A-LexAGAD into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-LexAGAD transgene.

    Depositor

    Chun Han

    Article

    Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

    Insert

    T2A-LexAGAD
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLX302 LacZ-V5 puro

    Plasmid

    #98579

    Purpose

    Lentiviral vector for constitutive expression of human LacZ with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    2x V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC118-FLIP-Puro

    Plasmid

    #84538

    Purpose

    a mock vector for generating FLIP targeting vectors

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

    Insert

    FLIP - Puro
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pHACK(Gal4)-DONR(T2A-Flp1)

    Plasmid

    #194770

    Purpose

    To insert T2A-Flp1 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Flp1 transgene.

    Depositor

    Chun Han

    Article

    Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

    Insert

    T2A-Flp1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWJ68

    Plasmid

    #46571

    Purpose

    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    w-dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    rpoZ

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mAzami Green-N1 (mAG)

    Plasmid

    #54798

    Purpose

    Localization: N1 Cloning Vector, Excitation: 492, Emission: 505

    Depositor

    Atsushi Miyawaki, Michael Davidson

    Article

    Karasawa et al J Biol Chem. 2003 Sep 5. 278(36):34167-7

    Type

    Empty backbone

    Use

    Tags

    mAzami Green

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

17. CrBlue-mcherry - pcDNA3.1

    Plasmid

    #64204

    Purpose

    CrBlue fused to mcherry on the C terminus

    Depositor

    Narasimhan Gautam

    Article

    Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    CrBlue
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pBabe NEO Venus

    Plasmid

    #98396

    Purpose

    Retroviral vector for constitutive Venus expression

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    Venus
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEINO5

    Plasmid

    #179489

    Purpose

    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

    Depositor

    Bogumil Karas

    Article

    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

    Insert

    aacC1
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-EF1a-MinE-IRES-Blast

    Plasmid

    #213115

    Purpose

    Expression of untagged MinE in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE (minE )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge