We narrowed to 2,141 results for: Pam

1. pAAV-hSyn-GRAB_DA1m

   Plasmid

   #113049

   Purpose

   express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

   Has Service

   AAV9

   Insert

   GPCR activation based DA sensor GRAB_DA1m (DRD2 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-GRAB_DA1h

   Plasmid

   #113050

   Purpose

   express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

   Has Service

   AAV Retrograde and AAV9

   Insert

   GPCR activation based DA sensor GRAB_DA1h (DRD2 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Phage-PGK-Flag-HA-FKBP-T(WT)

   Plasmid

   #181734

   Purpose

   for lentiviral expression of brachyury ORF in mammalian cells

   Depositor

   Charles Lin

   Article

   Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

   Insert

   T (brachyury)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   a silent mutation in the PAM site of an sgRNAt ta…

   Promoter

   Available since

   March 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. peSCBE3-NG-HF1-Hypa

   Plasmid

   #218166

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-HF1-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lenti-Cas9-VQR-Blast

   Plasmid

   #87155

   Purpose

   lentiviral expression of SpCas9-VQR (NGA PAM restricted)

   Depositor

   Daniel Bauer

   Article

   Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

   Insert

   SpCas9-VQR(D1135V,R1335Q,T1337R)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D1135V,R1335Q,T1337R

   Promoter

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMXS-IRES-BLAST flag-CAD hCPS1 AD

   Plasmid

   #188130

   Purpose

   Expresses C-terminal flag-tagged human CAD hCPS1 AD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMXS-IRES-BLAST flag-CAD delta Loop1 and Loop2

   Plasmid

   #169833

   Purpose

   Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 and F1366 - P1376 in allosteric domain

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Deletions of amino acids R1398 - S1407 and F1366 …

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMXS-IRES-BLAST flag-CAD delta Allosteric Domain

   Plasmid

   #169835

   Purpose

   Expresses C-terminal flag-tagged CAD with substitution of allosteric domain F1308 - C1455 with a (GGGS)X3 linker

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Substitution of amino acids F1308 - C1455 with a …

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMXS-IRES-BLAST flag-CAD 3R-3E

   Plasmid

   #188148

   Purpose

   Expresses C-terminal flag-tagged human CAD 3R-3E in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXS-IRES-BLAST flag-CAD hCPS1 TL

    Plasmid

    #188150

    Purpose

    Expresses C-terminal flag-tagged human CAD hCPS1 TL in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

11. pMXS-IRES-BLAST flag-CAD R1398A R1403A R1404A

    Plasmid

    #188134

    Purpose

    Expresses C-terminal flag-tagged human CAD R1398A R1403A R1404A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXS-IRES-BLAST flag-CAD R1403A R1404A

    Plasmid

    #188135

    Purpose

    Expresses C-terminal flag-tagged human CAD R1403A R1404A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXS-IRES-BLAST flag-CAD E1367A E1368A

    Plasmid

    #188143

    Purpose

    Expresses C-terminal flag-tagged human CAD E1367A E1368A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXS-IRES-BLAST flag-CAD E1367A E1368A E1373A

    Plasmid

    #188144

    Purpose

    Expresses C-terminal flag-tagged human CAD E1367A E1368A E1373A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXS-IRES-BLAST flag-CAD P1375A P1376A

    Plasmid

    #188146

    Purpose

    Expresses C-terminal flag-tagged human CAD P1375A P1376A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXS-IRES-BLAST flag-CAD 3E-3R

    Plasmid

    #188147

    Purpose

    Expresses C-terminal flag-tagged human CAD 3E-3R in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXS-IRES-BLAST flag-CAD G-Loop1

    Plasmid

    #188128

    Purpose

    Expresses C-terminal flag-tagged human CAD G-Loop1 in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXS-IRES-BLAST flag-CAD G-Loop2

    Plasmid

    #188129

    Purpose

    Expresses C-terminal flag-tagged human CAD G-Loop2 in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLEX307_CR-PDHA2S291D

    Plasmid

    #115200

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S291D (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLEX307_CR-PDHA2S293D

    Plasmid

    #115201

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S293D (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge