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Plasmid#206429PurposeA vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCHAP3_Sap
Plasmid#206849PurposeA plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUGW-VGAT-pHluorin
Plasmid#207467PurposeLentiviral expression of VGAT-pHluorinDepositorArticleInsertVGAT (Slc6a12 Rat)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CA-FLEX-RG
Plasmid#38043DepositorInsertRabies virus glycoprotein
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCHAP4
Plasmid#206850PurposeA plasmid containing a 16,121 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPt_Cp
Plasmid#206855PurposeA plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. The marker URA3 was integrated into the genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-nGFPMNase
Plasmid#187826Purposeexpress nGFPMNase protein in E coliDepositorInsertnGFPMNASE
UseTagshis tagExpressionBacterialMutationPromoterT7 PromotorAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed
Plasmid#226590PurposePharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.DepositorInsertPSAML-141F,Y115F-GlyR
UseAAVTagsP2A-Fusion RedExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pML31
Plasmid#203343Purpose3 input AND circuit on pLW555 backbone: PTet-P1, PLux-A1, PIcaRA:PPhlF:PAmtR-Q2, PTac-I1DepositorInsertsphlF
amtR
qacR
icaRA
UseTagsExpressionMutationPromoterPLux, PPhlF, PIcaRA, and PAmtR, PTac, and PTetAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINTO_7/8
Plasmid#206431PurposeA vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP2
Plasmid#206847PurposeA plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP1
Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pGEN-mSmoA1
Plasmid#37674DepositorInsertSmo (Smo Mouse)
UseTagsExpressionMammalianMutationW539LPromoterCMVAvailable sinceNov. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pCHAP8
Plasmid#206857PurposeA plasmid containing a 11,944 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP7
Plasmid#206853PurposeA plasmid containing a 13,748 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPt_Sap
Plasmid#206856PurposeA plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. It contains a single SapI site located in the URA3 marker, which was integrated into the genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP6
Plasmid#206852PurposeA plasmid containing a 15,671 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP5
Plasmid#206851PurposeA plasmid containing a 17,639 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only