Addgene

We narrowed to 2,370 results for: buc

Showing: 1061 - 1080 of 2370 results

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pCRIS-PITChv2-dTAG-BSD (BRD4)

Plasmid

#91795

Purpose

PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

Depositor

Behnam Nabet, James Bradner

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

FKBP_F36V-2xHA-P2A-BSD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promotorless

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMHE-miRFP-MAP4-MTBD

Plasmid

#171487

Purpose

T7 promotor drives in vitro transcription of miRFP-tagged mouse MAP4-Microtubule Binding Domain mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

MAP4-MTBD (Map4 Mouse)

Use

Pgehme

Tags

miRFP

Expression

Mutation

Aminoacids 658-1126

Promoter

T7

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHLS-EF1a-FKBP12-Gag(HIV)

Plasmid

#138476

Purpose

Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

HIV Gag

Use

Tags

FRBP12 and Lynn Myristolation site

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_TCF3_PBX1

Plasmid

#205937

Purpose

Express mEGFP-tagged fusion protein, TCF3_PBX1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TCF3_PBX1 (TCF3, PBX1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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PL-5LTR-RGR(DMD#1)-AmCyan-A

Plasmid

#138482

Purpose

Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Ribozyme-flanked gRNA and AmCyan

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_3xFLAG_V5_ mmDdx58_V5

Plasmid

#160108

Purpose

Expresses murine Ddx58 in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Ddx58 (Rigi Mouse)

Use

Lentiviral

Tags

3xFLAG, V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-HT-LC3-SV40-Hygro

Plasmid

#182045

Purpose

Lentiviral expression vector encoding HaloTag-LC3 for monitoring autophagosome closure

Depositor

Hong-Gang Wang

Article

Takahashi et al J Cell Biol. 2019 Oct 7;218(10):3336-335

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Lentiviral

Tags

HaloTag-TEV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_MYC_MYC

Plasmid

#205862

Purpose

Express mEGFP-tagged fusion protein, MYC_MYC from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

MYC_MYC (MYC Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

Plasmid

#207805

Purpose

E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.

Depositor

Koen Breyne

Article

Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do

Insert

E-NoMi

Use

Lentiviral

Tags

3xFlag tag, copGFP, mCherry, and nanoluciferase

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pESF140_HisSpy_DDB1∆B

Plasmid

#124213

Purpose

Insect cell expression vector for His6, Spy tagged hsDDB1∆B

Depositor

Eric Fischer

Article

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

DNA damage-binding protein 1 (DDB1 Human)

Use

Tags

His6-Spy

Expression

Insect

Mutation

internal deletion of BPB domain to aid crystalliz…

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PTEN_RPL11

Plasmid

#205903

Purpose

Express mEGFP-tagged fusion protein, PTEN_RPL11 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PTEN_RPL11 (PTEN, RPL11 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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YA1529: pAAV_hSyn-QuasAr3-P2A-CheRiff

Plasmid

#107700

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr3-P2A-CheRiff

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin I

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR del52-FLAG

Plasmid

#204523

Purpose

Retroviral expression of human CALR del52-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR del52-FLAG (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

del52

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR ins5-FLAG

Plasmid

#204524

Purpose

Retroviral expression of human CALR ins5-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5-FLAG (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

ins5

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223.1_mmDdx58 (closed)

Plasmid

#160107

Purpose

Expresses murine Ddx58 in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Ddx58 (Rigi Mouse)

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mScarlet-MAP4-MTBD

Plasmid

#171488

Purpose

T7 promotor drives in vitro transcription of mScarlet-tagged mouse MAP4-Microtubule Binding Domain mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

MAP4-MTBD (Map4 Mouse)

Use

Pgehme

Tags

mScarlet

Expression

Mutation

Aminoacids 658-1126

Promoter

T7

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1a-BFPCDK6-W

Plasmid

#208756

Purpose

Lentiviral vector expressing BFP fused with CDK6 cDNA

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_DOC2B_USP43

Plasmid

#205806

Purpose

Express mEGFP-tagged fusion protein, DOC2B_USP43 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

DOC2B_USP43 (DOC2B, USP43 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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YA1627: pAAV_hSyn-Dio-paQuasAr3-s-P2A-CheRiff-s

Plasmid

#107704

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

paQuasAr3-P2A-CheRiff

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_TRIO_TERT

Plasmid

#205946

Purpose

Express mEGFP-tagged fusion protein, TRIO_TERT from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TRIO_TERT (TRIO, TERT Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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