We narrowed to 15,937 results for: form

1. pH-mRuby2

   Plasmid

   #176547

   Purpose

   Expression of mRuby2 for auxotrophic selection in the absence of histidine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   yomRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQCXIP EGFP-ER [TM(SAC1)]

   Plasmid

   #186475

   Purpose

   Expression of the Endoplasmic Reticulum marker GFP-ER [TM(SAC1)] in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   SAC1 (SACM1L Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C-terminal transmembrane region of the protein: 5…

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AfrLEA3m_mCh-2xFKBP (pBS1137)

   Plasmid

   #185321

   Purpose

   For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   AfrLEA3m
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pST_APOE_HUMAN_E4_47-300 (pBS0847)

   Plasmid

   #185279

   Purpose

   For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   APOE_HUMAN_E4_47-300
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pST_PvLEA4_repeats_k3_26 (pBS0762)

   Plasmid

   #185244

   Purpose

   For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   PvLEA4_repeats_k3_26
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRetroX-TRE3G_NT-GSDMD_I105N_

   Plasmid

   #201174

   Purpose

   Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D N-terminal domain (Gsdmd Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   aa1-276 only, I105N

   Promoter

   TRE3G

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-CHRFAM7ADelta2bp

   Plasmid

   #62515

   Purpose

   mammalian expression of human duplicated Alpha7 with 2 bp deletion (CHRFAM7A-∆2bp)

   Depositor

   Henry Lester, Sherry Leonard

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   CHRFAM7ADelta2bp (CHRFAM7A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partially duplication of CHRNA7 with 2-bp deletio…

   Promoter

   CMV

   Available since

   March 20, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pMXs-puro HA-Atg14*dCC

   Plasmid

   #38266

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

   Insert

   autophagy related genes 14 (ATG14 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   changed A618T, A621T, C624A and T627C for siRNA r…

   Promoter

   LTR

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

9. mEmerald-MyosinIIC-C-18

   Plasmid

   #54194

   Purpose

   Localization: Cytoskeleton, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

   Insert

   MyosinIIC
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pST_SERF2_HUMAN (pBS0751)

    Plasmid

    #185239

    Purpose

    For the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    SERF2_HUMAN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1

    Plasmid

    #192471

    Purpose

    Transient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS12
    

    Use

    Tags

    AcGFP1

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAHS2parrc-mCh-2xFKBP (pBS1109)

    Plasmid

    #185304

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2parrc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pST_MAHS_RAMVA (pBS0310)

    Plasmid

    #185169

    Purpose

    For the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    MAHS_RAMVA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAcGFP1-N1-RvCAHS8-GS

    Plasmid

    #192461

    Purpose

    Expression of RvCAHS8-GS_linker-AcGFP1 in mammalian cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS8
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTriEx4-ADCY6-C2 (917-1165 aa)

    Plasmid

    #113904

    Purpose

    His-S tagged cytoplasmic catalytic domain 2 of Adenylate Cyclase 6

    Depositor

    Anjaparavanda Naren

    Article

    Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

    Insert

    Adenylate Cyclase 6 cytoplasmic catalytic domain 2 (ADCY6 Human)
    

    Use

    Tags

    His-S

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Please see Depositor Comments

    Promoter

    T7, CMV

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTriEx4-ADCY6-C1(306-672 aa)

    Plasmid

    #113903

    Purpose

    His-S tagged cytoplasmic catalytic domain 1 of Adenylate Cyclase 6

    Depositor

    Anjaparavanda Naren

    Article

    Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

    Insert

    Adenylate Cyclase 6 cytoplasmic catalytic domain 1 (ADCY6 Human)
    

    Use

    Tags

    His-S

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7, CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKTop::tse5-CT (1169-1281)

    Plasmid

    #192955

    Purpose

    For membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    tse5-CT (1169-1281)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1281)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ApoE2_mCh-SspB (pBS1142)

    Plasmid

    #185324

    Purpose

    For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pST_MAHS_RAMVA_81-236-FLAG (pBS0297)

    Plasmid

    #185328

    Purpose

    For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    MAHS_RAMVA_81-236-FLAG
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFOSGE_COF4C03

    Plasmid

    #70961

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    His2Av (His2Av Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only