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pEF1α-woolly-monkey-retroCHMP3(148)-HA

Plasmid

#154250

Purpose

expresses prematurely truncated woolly monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of retroCHMP3 at amino acid…

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α-pygmy-marmoset-retroCHMP3(148)-HA

Plasmid

#154251

Purpose

expresses prematurely truncated pygmy marmoset retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of retroCHMP3 at amino acid…

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-H2B-Sirius

Plasmid

#175837

Purpose

Express Sirius with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with nucleus-targeting sequence

Tags

H2B (human histone 2B)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Sirius-KRasCT

Plasmid

#175831

Purpose

Express Sirius with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-SECFP-KRasCT

Plasmid

#175832

Purpose

Express SECFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-EGFP

Plasmid

#174477

Purpose

Express EGFP with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with actin-targeting sequence

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-SECFP-Sec61β

Plasmid

#174465

Purpose

Express SECFP with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with endoplasmic reticulum-targeting sequence

Tags

Sec61 subunit beta

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-iRFP-KRasCT

Plasmid

#175836

Purpose

Express iRFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-SECFP

Plasmid

#174476

Purpose

Express SECFP with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with actin-targeting sequence

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-Sirius

Plasmid

#174475

Purpose

Express Sirius with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with actin-targeting sequence

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Tom20-Sirius

Plasmid

#174184

Purpose

Express Sirius with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Sirius-Sec61β

Plasmid

#174464

Purpose

Express Sirius with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with endoplasmic reticulum-targeting sequence

Tags

Sec61 subunit beta

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-GalT-Sirius

Plasmid

#174469

Purpose

Express Sirius with golgi apparatus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with golgi apparatus-targeting sequence

Tags

β1,4-galactose transferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-0-mTagBFP2(11x7)

Plasmid

#172064

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 0 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBN216

Plasmid

#167963

Purpose

express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y713E, Y715E, Y717E, Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN217

Plasmid

#167964

Purpose

express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y708E, Y713E, Y715E, Y717E, Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN262

Plasmid

#167967

Purpose

express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y708E, Y713E, Y715E, Y717E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBRC901

Plasmid

#167948

Purpose

express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

Tags

hexa-histidine tag

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN202

Plasmid

#167950

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y713E, Y715E, Y717E, Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJS697

Plasmid

#168778

Purpose

Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Type

Empty backbone

Tags

Cmyc and HA

Expression

Yeast

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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