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pSMART-HCKan-yibDp-VHH-GFPminimizer

Plasmid

#202469

Purpose

Expresses the nanobody GFP minimizer after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP minimizer

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-roGFP2

Plasmid

#202462

Purpose

Expresses redox sensitive GFP (roGFP) after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13210

Plasmid

#219885

Purpose

The base plasmid of TUNEYALI for TF20

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF20 (YALI1_D16173g) and homologous arm matching TF20

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-HMP1_FABD

Plasmid

#186436

Purpose

Expresses the HMP1 F-actin Binding domain (677-927) with an N-terminal GST tag and a PRescission Protease cleavage site

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

Insert

HMP1 (F-actin Binding Domain) (hmp-1 Nematode)

Tags

GST

Expression

Bacterial

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAMC042z21

Plasmid

#209747

Purpose

RepA(N99D) SC101 T0-BS7 proD SD8 sfGFP( V93AGGA Y151UAGA) ProK 5 tRNA operon tetA-orfL bla

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

sfGFP - tRNA operon

Expression

Bacterial

Mutation

sfGFP - Valine 93 AGGA quadruplet codon, Tyrosine…

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-drAgo2

Plasmid

#221711

Purpose

Expression of zebrafish Ago2 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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p8189 pHAGE N-V5 PTPN14 C1121S

Plasmid

#221648

Purpose

Constitutive ectopic expression of human PTPN14 C1121S mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 C1121S (PTPN14 Human)

Use

Lentiviral

Tags

V5

Mutation

C1121S mutation at the putative catalytic site

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEY142

Plasmid

#223489

Purpose

unc-17(3k) fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-17(3k) (unc-17 Nematode)

Expression

Worm

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY136

Plasmid

#223483

Purpose

tag-168 fluorescent neural reporter driving nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tag-168

Expression

Worm

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY120

Plasmid

#223467

Purpose

sra-11(pB) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

sra-11(pB) (sra-11 Nematode)

Expression

Worm

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY118

Plasmid

#223465

Purpose

ser-2(prom3) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ser-2(prom3) (ser-2 Nematode)

Expression

Worm

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY114

Plasmid

#223461

Purpose

pdf-1 fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

pdf-1 (pdf-1 Nematode)

Expression

Worm

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY110

Plasmid

#223457

Purpose

odr-10 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

odr-10 (odr-10 Nematode)

Expression

Worm

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY100

Plasmid

#223447

Purpose

lad-2 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

lad-2 (lad-2 Nematode)

Expression

Worm

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY76

Plasmid

#223445

Purpose

dop-2 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

dop-2 (dop-2 Nematode)

Expression

Worm

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY74

Plasmid

#223444

Purpose

cho-1(3.1-2.6k) fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

cho-1(3.1-2.6k) (cho-1 Nematode)

Expression

Worm

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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SUMO-LPQTG-pET

Plasmid

#221851

Purpose

Expresses SUMO-2 with sortase motif and His tag

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

SUMO-2 (SUMO2 Human)

Expression

Bacterial

Mutation

Gln88Leu, Gln89Pro

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPU-HSA-miR30-MDA5

Plasmid

#174222

Purpose

All-in-one control rescue-MDA5 shRNA knockdown vector

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

HSA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1312-AAV-EFSNC-dSaCas9-KRAB

Plasmid

#223157

Purpose

Expression of KRAB with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEY135

Plasmid

#223482

Purpose

tab-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tab-1 (tab-1 Nematode)

Expression

Worm

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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