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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 10801 - 10820 of 23077 results

  2. pKP1693

    Plasmid
    #167595
    Purpose
    Express FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Gresock et al J Bacteriol. 2017 Apr 25;199(10). pii: J
    Insert
    FepA
    Expression
    Bacterial
    Mutation
    His6 tag inserted after residue 393 in the mature…
    Available Since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167595-plasmid-map-sequence-id-332904

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  3. pKB235

    Plasmid
    #170024
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    CCL20 (CCL20 Human, Synthetic, Escherichia coli)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170024-plasmid-map-sequence-id-336757

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  4. pRWJB006

    Plasmid
    #160113
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial and Plant
    Promoter
    35S
    Available Since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160113-plasmid-map-sequence-id-325470

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  5. pRWJB004

    Plasmid
    #160112
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial and Plant
    Promoter
    UBQ10
    Available Since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160112-plasmid-map-sequence-id-325469

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  7. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted the…
    Promoter
    T7
    Available Since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160434-plasmid-map-sequence-id-318954

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  8. pCDF-NtXNtS1HR1AtR2NtB2

    Plasmid
    #160890
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-S1H
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    rbcS-SH1: T7, chaperones: T13
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160890-plasmid-map-sequence-id-318865

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  10. pSWD95Y14D

    Plasmid
    #159098
    Purpose
    CckA FRET Sensor with cyclic-di-GMP point mutant
    Depositor
    Seth Childers
    Article
    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do
    Insert
    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
    Expression
    Bacterial
    Mutation
    Mutated Y514 to D514
    Promoter
    Xylose
    Available Since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159098-plasmid-map-sequence-id-310723

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  11. pSWD95D293A

    Plasmid
    #159099
    Purpose
    CckA FRET Sensor with DITE motif mutant
    Depositor
    Seth Childers
    Article
    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do
    Insert
    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
    Expression
    Bacterial
    Mutation
    CckA D293 mutated to A293 and V199I on mRuby3 (P…
    Promoter
    Xylose
    Available Since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159099-plasmid-map-sequence-id-316589

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  15. SMT201

    Plasmid
    #134816
    Purpose
    SMT101 with TET promoter for TYMS, in vivo assays
    Depositor
    Tanja Kortemme
    Article
    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134816-plasmid-map-sequence-id-274005

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Showing: 10801 - 10820 of 23077 results