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pCHKU30.1-2.2

Plasmid

#115532

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Expression

Yeast

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-Cα(S11A)-mPAGFP

Plasmid

#114134

Purpose

Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-CαN-mPAGFP

Plasmid

#114114

Purpose

Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-Mps1-KD

Plasmid

#114066

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

Insert

Mps1-KD (TTK Human)

Tags

GST

Expression

Insect

Mutation

Mps1 KD (D664A)

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-Mps1-WT

Plasmid

#114065

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

Insert

Mps1-WT (TTK Human)

Tags

GST

Expression

Insect

Mutation

none

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS2235

Plasmid

#111888

Purpose

High copy number plasmid containing Ple320 (POU4F1 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple320 (POU4F1 Human)

Use

Unspecified

Promoter

Ple320 (POU4F1 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2236

Plasmid

#111889

Purpose

High copy number plasmid containing Ple321 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple321 (TUBB3 Human)

Use

Unspecified

Promoter

Ple321 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCHSU65.2-2.2

Plasmid

#110678

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3

Expression

Yeast

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU65.1-2.2

Plasmid

#110677

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Tags

flag

Expression

Yeast

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU30.2-2.2

Plasmid

#110663

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTE3179

Plasmid

#107528

Purpose

Expresses Mb crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Mb crRNA
mCherry

Expression

Mammalian

Promoter

CBh and human U6

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS1930

Plasmid

#79779

Purpose

High copy number pUC vector containing Ple257 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple257

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple257 (PAX6 MiniPromoter)

Available Since

Nov. 15, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2047

Plasmid

#79655

Purpose

ssAAV genome with Ple258 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple258-EmGFP WPRE

Use

AAV

Promoter

PAX6 Enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS1931

Plasmid

#79780

Purpose

High copy number pUC vector containing Ple258 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple258

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple258 (PAX6 MiniPromoter)

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEMS1929

Plasmid

#79778

Purpose

High copy number pUC vector containing Ple256 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple256

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple256 (PAX6 MiniPromoter)

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1928

Plasmid

#79777

Purpose

High copy number pUC vector containing Ple255 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple255

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple255 (PAX6 MiniPromoter)

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1927

Plasmid

#79776

Purpose

High copy number pUC vector containing Ple254 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple254

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple254 (PAX6 MiniPromoter)

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pSicoR (EGFP) shPnky-2

Plasmid

#79142

Purpose

Stable expression of shRNA targeting mouse Pnky. The shRNA (and EGFP) can be excised by the addition of Cre

Depositor

Daniel Lim

Article

Ramos et al Cell Stem Cell. 2015 Apr 2;16(4):439-47.

Insert

shPnky-2 (Gm30731 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for the shRNA and CMV for EGFP

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2025

Plasmid

#49132

Purpose

AAV plasmid with NOV (Ple303) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple303-iCre

Use

AAV

Expression

Mammalian

Promoter

NOV

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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NKP91 GFP (1034)

Plasmid

#62023

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

Tmem41A (TMEM41A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only