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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,044 results for: Met

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Showing: 10841 - 10860 of 19044 results

  2. pQdCas12a.sggfp(A)

    Plasmid
    #236038
    Purpose
    The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236038-plasmid-map-sequence-id-469933

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  3. pQdCas12a.sggfp(B)

    Plasmid
    #236040
    Purpose
    The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236040-plasmid-map-sequence-id-469924

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  10. pXD80ENSF26-Pct3-(+)-Eldh1(nHis6)(GpDSM19378)

    Plasmid
    #233874
    Purpose
    Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification
    Depositor
    Emily Balskus
    Article
    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72
    Insert
    (+)-Eldh1 (GPA_25810 Gordonibacter pamelaeae DSM 19378)
    Tags
    His
    Expression
    Bacterial
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233874-plasmid-map-sequence-id-467490

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  15. pXD80ENSF27-Pct3-(+)-Eldh2(nHis6)(GpDSM19378)

    Plasmid
    #233875
    Purpose
    Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification
    Depositor
    Emily Balskus
    Article
    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72
    Insert
    (+)-Eldh2 (GPA_25800 Gordonibacter pamelaeae DSM 19378)
    Tags
    His
    Expression
    Bacterial
    Available Since
    March 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233875-plasmid-map-sequence-id-463816

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  17. Kif3A-EGFP-SNAP

    Plasmid
    #229852
    Purpose
    Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    William Hancock
    Insert
    Kif3A (Kif3a Mouse)
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229852-plasmid-map-sequence-id-460709

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  18. pCIFR-8

    Plasmid
    #227666
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Am) used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    AmR
    Expression
    Bacterial
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227666-plasmid-map-sequence-id-458899

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  20. JD180

    Plasmid
    #229486
    Purpose
    Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes
    Depositor
    Nerea Zabaleta
    Article
    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3
    Insert
    E2A, L4-22k/33k, mCitrine
    Use
    AAV and Adenoviral
    Expression
    Mammalian
    Promoter
    Tre (E2A, L4-22k/33k), ETR (mCitrine)
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    229486-plasmid-map-sequence-id-455846

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Showing: 10841 - 10860 of 19044 results