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pDEST17_A1LigN_EC

Plasmid

#22296

Depositor

Oscar Millet

Article

Tadeo et al PLoS Biol. 2009 Dec;7(12):e1000257. doi:

Insert

A1 domain NAD+ dependent DNA ligase N

Tags

His tag

Expression

Bacterial

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only
co GNLX/pcDNA3.1(+)

Plasmid

#253528

Purpose

Green bacterial luciferase in mammalian cells

Depositor

Takeharu Nagai

Article

Kusuma et al Proc Natl Acad Sci U S A. 2024 Oct 8;121

Insert

co GNLX

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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co RNLX/pcDNA3.1(+)

Plasmid

#253531

Purpose

Red bacterial luciferase in mammalian cells

Depositor

Takeharu Nagai

Article

Kusuma et al Proc Natl Acad Sci U S A. 2024 Oct 8;121

Insert

co RNLX

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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co CNLX/pcDNA3.1(+)

Plasmid

#253527

Purpose

Cyan bacterial luciferase in mammalian cells

Depositor

Takeharu Nagai

Article

Kusuma et al Proc Natl Acad Sci U S A. 2024 Oct 8;121

Insert

co CNLX

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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pJPC668

Plasmid

#253814

Purpose

Fluorescent reporter AmCyan

Depositor

Daniel Voytas

Article

Tibebu et al Front. Plant Sci. 17:1794888.

Insert

AmCyan

Use

Golden gate subcloning vector

Promoter

none

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAP696e

Plasmid

#246042

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Article

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Linker::T2A::Linker::mNeonGreen::Linker+CAAX

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pAP697e

Plasmid

#253944

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Article

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Linker::T2A::Linker::mScarlet::Linker+CAAX

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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S’RP + PodJ’

Plasmid

#241365

Purpose

SpmX△TM-mRFP-PopZ, sfGFP-PodJ249pse

Depositor

Hsiao-Chun Huang

Article

Chou et al Commun Biol. 2025 Jul 20;8(1):1078. doi:

Inserts

SpmX△TM-mRFP-PopZ
sfGFP-PodJ249pse

Expression

Bacterial

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_lacI_Plac

Plasmid

#243920

Purpose

GoldenBraid2.0 IPTG/Lactose-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

lacI and PlacZYA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_tetR_Ptet

Plasmid

#243924

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetR and PtetA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_cI857_PR

Plasmid

#243926

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cI857 and PR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_PT7

Plasmid

#243928

Purpose

GoldenBraid2.0 T7 RNA polymerase-dependent promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_PT7lac

Plasmid

#243929

Purpose

GoldenBraid2.0 T7 RNA polymerase and IPTG/Lactose-dependent promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7lac

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_lacI_Plac

Plasmid

#243909

Purpose

GoldenBraid2.0 IPTG/Lactose-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

lacI and PlacZYA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_araC_Para

Plasmid

#243911

Purpose

GoldenBraid2.0 Arabinose-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

araC and ParaBAD

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_tetR_Ptet

Plasmid

#243913

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetR and PtetA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_cI857_PR

Plasmid

#243915

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cI857 and PR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_PT7

Plasmid

#243917

Purpose

GoldenBraid2.0 T7 RNA polymerase-dependent promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_PT7lac

Plasmid

#243918

Purpose

GoldenBraid2.0 T7 RNA polymerase and IPTG/Lactose-dependent promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7lac

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pJL1-sFLAG-p5-PRB4_R7-9-FETCH1

Plasmid

#210651

Purpose

In vitro expression of polyProline FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

sFLAG-p5-PRB4_R7-9-FETCH1

Use

Synthetic Biology

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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