We narrowed to 15,981 results for: LEA

1. NES-mCherry-P4Mx1

   Plasmid

   #108143

   Purpose

   PI4P biosensor

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_Lyn11-HaloTag7

   Plasmid

   #175533

   Purpose

   Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lyn11-HaloTag7
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. MR015-H11-L2-TALEN

   Plasmid

   #51554

   Purpose

   Expresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.

   Depositor

   Michele Calos

   Article

   Zhu et al Nucleic Acids Res. 2013 Dec 4.

   Insert

   H11-L2 TALEN
   

   Use

   TALEN

   Expression

   Mammalian

   Available Since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-BAF

   Plasmid

   #172447

   Purpose

   Expression of nuclear protein BAF with an N-terminal mCherry tag

   Depositor

   Stephen Royle

   Article

   Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   BAF
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MR015-H11-R2-TALEN

   Plasmid

   #51555

   Purpose

   Expresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).

   Depositor

   Michele Calos

   Article

   Zhu et al Nucleic Acids Res. 2013 Dec 4.

   Insert

   H11-R2 TALEN
   

   Use

   TALEN

   Expression

   Mammalian

   Available Since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-OTI-TCRB_p2_TCRA

   Plasmid

   #131034

   Purpose

   Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

   Depositor

   Jeff Hammerbacher

   Article

   Aksoy et al bioRxiv

   Inserts

   TCRB (Tcrb Mouse)
   TCRA (Tcra Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV/T7

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-3C-sGFP11_pcDNA3.1(-)

   Plasmid

   #182932

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Tags

   3C-cleavable split GFP 11, FLAG tag, and HA signa…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2xMARS

   Plasmid

   #205233

   Purpose

   Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells

   Depositor

   Jeremy Baskin

   Insert

   two repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
   

   Tags

   Nuclear Export Sequence and mScarlet-i

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNH-NanoLuc

   Plasmid

   #173075

   Purpose

   Expression vector for E. coli producing target protein with N-terminal Nanoluciferase fusion

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-GR-ICUE2

    Plasmid

    #173017

    Purpose

    Improved green-red FRET based cAMP indicator.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    GR-ICUE2
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAPV-DsRed2-ER-5

    Plasmid

    #170570

    Purpose

    Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    APV-DsRed2-ER-5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR4-myc-nuc (w556-1)

    Plasmid

    #20026

    Purpose

    Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Gateway entry vector

    Tags

    Myc and NLS

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-3C-sGFP1-10_pcDNA3.1(-)

    Plasmid

    #182931

    Purpose

    For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

    Depositor

    William Weis

    Article

    Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

    Type

    Empty backbone

    Tags

    3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. mini-donor - SOX17-EGFP

    Plasmid

    #206048

    Purpose

    this mini-donor knockin vector can be used to knock EGFP into SOX17 locus

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    nuclear EGFP
    

    Use

    Knockin donor vector

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21a - TR

    Plasmid

    #89621

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Thioredoxin
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-Universal

    Plasmid

    #52694

    Purpose

    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

    Depositor

    Sebastian Lourido

    Inserts

    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    

    Use

    CRISPR; Toxoplasma gondii

    Tags

    3X FLAG and Nuclear localization signal

    Promoter

    TgTUB1 and Toxoplasma U6

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. FnR8-10

    Plasmid

    #182569

    Purpose

    Human fibronectin fragment, type-III repeats 8-10, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 8-10
    

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1379A

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Parp14-c012

    Plasmid

    #173099

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP14 (PARP14 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    W1003-A1190

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. PEX-LV

    Plasmid

    #12120

    Depositor

    Inder Verma

    Article

    Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

    Insert

    Matrix Metalloproteinase 2 (Mmp2 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    noncatalytic fragment of MMP2 comprised of the fi…

    Available Since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

20. NES-EGFP-P4Mx1

    Plasmid

    #108121

    Purpose

    PI4P biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    X.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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