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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 20,331 results for: Sele

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Showing: 10941 - 10960 of 20331 results

  2. pcDNA3-Lyn-PlcR(K30/32L)

    Plasmid
    #181831
    Purpose
    Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.
    Depositor
    Jin Zhang
    Publication
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-PlcR (K30L,K32L)
    Tags
    ECFP, EYFP, and N-terminal targeting sequence fro…
    Expression
    Mammalian
    Mutation
    Lysines 30 and 32 in PH domain mutated to leucine.
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181831-plasmid-map-sequence-id-364248

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  3. pcDNA3.1(+)-mCherry-p115(S240A)

    Plasmid
    #181997
    Purpose
    Mammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.
    Depositor
    Jin Zhang
    Publication
    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-p115(S240A) (ARHGEF1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Serine 240 changed to alanine in p115.
    Promoter
    CMV
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181997-plasmid-map-sequence-id-362770

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  4. pPHR-mCherry-hHP1alpha

    Plasmid
    #181884
    Purpose
    Soluble light-dependent effector protein (human HP1alpha) for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Publication
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PHR-mCherry-hHP1alpha (CBX5 Human, Mustard Weed)
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    181884-plasmid-map-sequence-id-359625

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  5. Epac-S-H30

    Plasmid
    #170337
    Purpose
    CFP(UST)_EPACdDEPCD_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CFP(UST)_EPACdDEPCD_Y(UST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170337-plasmid-map-sequence-id-344639

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  7. pAuroraA GFP-AURKA-GFP

    Plasmid
    #157765
    Purpose
    Expression of AURKA fused to GFP at both ends in mammalian cells under AurkA promoter
    Depositor
    Marc Tramier
    Publication
    Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AURKA (AURKA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    promoter CMV replaced by AurkA promoter
    Promoter
    AURKA minimal
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    157765-plasmid-map-sequence-id-310867

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  8. pCytERM-mGreenLantern

    Plasmid
    #164467
    Purpose
    Visualization of the ER in cells (compatible with OSER assay)
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mGreenLantern
    Tags
    Cytochrome p450 first 29 animo acids (Oryctolagus…
    Expression
    Mammalian
    Mutation
    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…
    Promoter
    CMV
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    164467-plasmid-map-sequence-id-322379

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  10. Venus2-DeltaCT-STAT3

    Plasmid
    #123179
    Purpose
    Expresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Publication
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    Tags
    Venus2 (aa 158-238) for bimolecular fluorescence …
    Expression
    Mammalian
    Mutation
    Deletion of the SH2 and TAD domains (C-terminus, …
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123179-plasmid-map-sequence-id-238651

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  12. pcDNA3-FKBP-ICUE3-NLS

    Plasmid
    #138255
    Purpose
    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Jin Zhang
    Publication
    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP-ICUE3-NLS
    Tags
    FKBP, Nuclear localization signal (NLS), cpVenus[…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    138255-plasmid-map-sequence-id-307462

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  13. pcDNA3-mCherry-mCherry-FLARE-Cameleon

    Plasmid
    #123352
    Purpose
    Red-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-mCherry-FLARE-Cameleon
    Tags
    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    123352-plasmid-map-sequence-id-307178

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  14. pcDNA3-Venus-Venus-FLARE-Cameleon

    Plasmid
    #123351
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus-Venus-FLARE-Cameleon
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123351-plasmid-map-sequence-id-307106

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  15. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

    Plasmid
    #123339
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus-cpVenus-FLARE-EKAR-EV
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123339-plasmid-map-sequence-id-307068

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  16. pcDNA3-mCherry-mCherry-FLARE-AKAR

    Plasmid
    #123333
    Purpose
    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-mCherry-FLARE-AKAR
    Tags
    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    123333-plasmid-map-sequence-id-306922

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  17. pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

    Plasmid
    #140014
    Purpose
    Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Publication
    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021
    Insert
    Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Y126A, Y197F
    Promoter
    EF1
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140014-plasmid-map-sequence-id-297743

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  19. pcDNA3-sACE2v5-sfGFP

    Plasmid
    #145176
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 5
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145176-plasmid-map-sequence-id-292575

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  20. pcDNA3-sACE2v4-sfGFP

    Plasmid
    #145175
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 4
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145175-plasmid-map-sequence-id-292620

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Showing: 10941 - 10960 of 20331 results